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anti-CACNB4 antibody :: Goat CACNB4 Polyclonal Antibody

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Catalog # MBS421064
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

CACNB4, Polyclonal Antibody

Also Known As

Goat anti-CACNB4 (C terminus) Antibody

Product Synonym Names
Goat Anti-CACNB4 (internal) Antibody; CACNB4; CAB4; CACNLB4; calcium channel, voltage-dependent, beta 4 subunit; dihydropyridine-sensitive L-type, calcium channel beta-4 subunit; EA5; EIG9; EJM; EJM4; EJM6; OTTHUMP00000207247; OTTHUMP00000207249; CACNB4 antibody; CAB4 antibody; CACNLB4 antibody; calcium channel; voltage-dependent; beta 4 subunit antibody; dihydropyridine-sensitive L-type; calcium channel beta-4 subunit antibody; CACNB4 (C terminus)
Antibody/Peptide Pairs
CACNB4 peptide (MBS428193) is used for blocking the activity of CACNB4 antibody (MBS421064)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
486
Immunogen Sequence
CSPGGYSHDS RHRL
OMIM
601949
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CSPGGYSHDSRHRL, from the C Terminus of the protein sequence according to NP_001005747.1; NP_000717.2; NP_001005746.1.
Epitope
C Terminus
Note
This antibody is expected to recognise all reported protein isoforms of human CACNB4 (NP_001005747.1; NP_000717.2; NP_001005746.1;)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CACNB4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CACNB4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CACNB4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 60+55kDa bands observed in Human Bone Marrow lysates and in transfected HEK293 transiently expressing CACNB4 (calculated MW of 58.2kDa according to NP_000717.2 and 54.7kDa according to NP_001005747.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-CACNB4 antibody
(0.2ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CACNB4 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CACNB4 antibody
HEK293 overexpressing CACNB4 (RC210440) and probed (mock transfection in first lane), tested by Origene.
anti-CACNB4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CACNB4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_000717.2; NP_001005746.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,331 Da
NCBI Official Full Name
voltage-dependent L-type calcium channel subunit beta-4 isoform a
NCBI Official Synonym Full Names
calcium voltage-gated channel auxiliary subunit beta 4
NCBI Official Symbol
CACNB4  [Similar Products]
NCBI Official Synonym Symbols
EA5; EJM; CAB4; EIG9; EJM4; EJM6; CACNLB4
  [Similar Products]
NCBI Protein Information
voltage-dependent L-type calcium channel subunit beta-4
UniProt Protein Name
Voltage-dependent L-type calcium channel subunit beta-4
UniProt Synonym Protein Names
Calcium channel voltage-dependent subunit beta 4
UniProt Gene Name
CACNB4  [Similar Products]
UniProt Synonym Gene Names
CACNLB4; CAB4  [Similar Products]
UniProt Entry Name
CACB4_HUMAN
NCBI Summary for CACNB4
This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for CACNB4
CACNB4: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB4 are the cause of susceptibility to epilepsy, idiopathic generalized type 9 (EIG9). A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. Defects in CACNB4 are the cause of susceptibility to juvenile myoclonic epilepsy type 6 (EJM6). EJM6 is a subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Defects in CACNB4 are the cause of episodic ataxia type 5 (EA5). EA5 is a disorder characterized by episodes of vertigo and ataxia that last for several hours. Interictal examination show spontaneous downbeat and gaze-evoked nystagmus, mild dysarthria and truncal ataxia. Belongs to the calcium channel beta subunit family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, calcium

Chromosomal Location of Human Ortholog: 2q22-q23

Cellular Component: cytosol; internal side of plasma membrane; plasma membrane; synapse; voltage-gated calcium channel complex

Molecular Function: calcium channel activity; high voltage-gated calcium channel activity; protein binding; protein kinase binding; voltage-gated calcium channel activity

Biological Process: action potential propagation; adult walking behavior; cAMP metabolic process; cellular calcium ion homeostasis; detection of light stimulus involved in visual perception; gamma-aminobutyric acid secretion; gamma-aminobutyric acid signaling pathway; membrane depolarization; muscle fiber development; neuromuscular junction development; Peyer's patch development; spleen development; synaptic transmission; synaptic transmission, glutamatergic; T cell receptor signaling pathway; thymus development; transport

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 9; Episodic Ataxia, Type 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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