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anti-PSME1 antibody :: Rabbit PA28, alpha Polyclonal Antibody

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Catalog # MBS611019
Unit / Price
  0.1 mg  /  $620 +1 FREE 8GB USB
anti-PSME1 antibody
Product Name

PA28, alpha (PSME1), Polyclonal Antibody

Full Product Name

PA28, alpha, CT (Proteasome Activator 28-alpha subunit)

Product Synonym Names
Anti -PA28, alpha, CT (Proteasome Activator 28-alpha subunit)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_000014.8 (24605378..24608176). Location: 14q11.2
OMIM
600654
3D Structure
ModBase 3D Structure for Q06323
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Macaque, Mouse, Porcine, Rat
Specificity
Reacts with the C-terminal region of mouse, rat, human, crab-eating macaque (monkey) and pig PA28a. On Western blots, it identifies the target band at ~28kD. Faint bands of unknown origin may be observed in some homogenate or lysate preparations.
Purity/Purification
Affinity Purified
Epitope-affinity purified from rabbit antiserum.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Immunogen
Synthetic peptide derived from the C-terminal region of the proteasome activator 28-alpha subunit (PA28a, also known as 11s regulator complex alpha subunit (REG-alpha) (11S REG) and Interferon gamma up-regulated I-5111 protein (IGUP I-5111) protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-PSME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PSME1 antibody
PA281 is a 180kD proteasome activator protein that is highly conserved among species.2,3 The PA28 complex is composed of two homologous subunits,a and b, which have similar catalytic properties and associate to form a hexameric ring4-5 that b binds to both ends of the 20S proteasome.6 PA28 is a strong proteasome activator; its affinity for the proteasome is about 10-fold greater than recombinant PA28b.5 The carboxy terminus of PA28a is necessary for PA28 to bind to and activate the proteasome.7 The activation of the proteasome by PA28 is an interferon-g (IFNg) inducible.8 PA28 is constitutively expressed by antigen presenting cells and is required for presentation of certain major histocompatibility (MHC) class I antigens.9-10 PA28 a/b can enhance antigen processing independently of changes in 20S proteasome subunit composition or assembly.11 Down-regulation of PA28 results in abnormal proteasome activation, and has been implicated in the development of intimal hyperplasia (IH) in animal models.6 Detection of anti-PA28a antibody is significantly higher in serum from patients with systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS), suggesting the importance of an antigen-driven system in the induction of an autoimmune response to PA28 complex.12 Impaired expression of proteasome subunits is involved in the loss of HLA class I expression in human colon cancer cells.13
Product Categories/Family for anti-PSME1 antibody
Applications Tested/Suitable for anti-PSME1 antibody
Western Blot (WB)
Application Notes for anti-PSME1 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1-3ug/ml
NCBI/Uniprot data below describe general gene information for PSME1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,723 Da[Similar Products]
NCBI Official Full Name
PA28 alpha subunit
NCBI Official Synonym Full Names
proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
NCBI Official Symbol
PSME1  [Similar Products]
NCBI Official Synonym Symbols
PA28A; IFI5111; MGC8628; REGalpha; PA28alpha
  [Similar Products]
NCBI Protein Information
proteasome activator complex subunit 1; REG-alpha; IGUP I-5111; OTTHUMP00000027975; OTTHUMP00000164702; 29-kD MCP activator subunit; interferon-gamma IEF SSP 5111; proteasome activator subunit-1; 11S regulator complex alpha subunit; 11S regulator complex subunit alpha; proteasome activator 28 subunit alpha; interferon-gamma-inducible protein 5111; interferon gamma up-regulated I-5111 protein; activator of multicatalytic protease subunit 1
UniProt Protein Name
Proteasome activator complex subunit 1
UniProt Synonym Protein Names
11S regulator complex subunit alpha; REG-alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; IGUP I-5111; Proteasome activator 28 subunit alpha
UniProt Gene Name
PSME1  [Similar Products]
UniProt Synonym Gene Names
IFI5111  [Similar Products]
UniProt Entry Name
PSME1_HUMAN
NCBI Summary for PSME1
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]
UniProt Comments for PSME1
PSME1: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers. By IFNG/IFN-gamma. Belongs to the PA28 family.

Protein type: Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome activator complex; cytoplasm; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-PSME1 antibody
 Reference Product  PubMed Publications
 PSME2 antibody  >47 publications with PSME1 and PSME2
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