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anti-YWHAB antibody :: Rabbit 14-3-3 beta/alpha Polyclonal Antibody

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Catalog # MBS125664
Unit / Price
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  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Western Blot (WB)
Product Name

14-3-3 beta/alpha (YWHAB), Polyclonal Antibody

Full Product Name

Rabbit polyclonal to Human 14-3-3 beta/alpha

Product Synonym Names
14-3-3 b/a; 14-3-3 b/a Rabbit pab Antibody; 14-3-3 beta/alpha Rabbit pab Antibody
Product Synonym Gene Name
YWHAB; GW128; HS1; KCIP-1; 14-3-3;[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_000020.10 (43514344..43537161). Location: 20q13.1
OMIM
601289
3D Structure
ModBase 3D Structure for P31946
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, mouse, rat
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
1mg/ml (lot specific)
Immunogen
A fusion protein of human 14-3-3 beta/alpha
Storage Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C
Other Notes
Small volumes of anti-YWHAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-YWHAB antibody
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau, and eta that have been identified in mammals. The initially described alpha and delta isoforms are confirmed to be phosphorylated forms of beta and zeta, respectively (3). Through their amino-terminal alpha helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
Applications Tested/Suitable for anti-YWHAB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-YWHAB antibody
WB: 1/500-1000; IHC: 1/50

Western Blot (WB) of anti-YWHAB antibody
Western blot analysis of extracts of Jurkat cells, using YWHAB antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
anti-YWHAB antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for YWHAB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,082 Da[Similar Products]
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
NCBI Official Symbol
YWHAB  [Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1
  [Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1
Protein Family
UniProt Gene Name
YWHAB  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433B_HUMAN
Product References and Citations for anti-YWHAB antibody
1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9.
2. Mackintosh, C. (2004) Biochem. J. 381, 329-42.
3. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875-84.
4. Yaffe, M.B. (2002) FEBS Lett. 513, 53-7.
5. Bridges, D. and Moorhead, G.B. (2004) Sci. STKE 2004, re10.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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