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anti-YWHAB antibody :: Rabbit 14-3-3 beta Polyclonal Antibody

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Catalog # MBS8247745
Unit / Price
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  0.05 mL  /  $195 +1 FREE 8GB USB
  0.1 mL  /  $250 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

14-3-3 beta (YWHAB), Polyclonal Antibody

Full Product Name

Anti-14-3-3 beta Antibody

Product Synonym Names
14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601289
3D Structure
ModBase 3D Structure for P31946
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of 14-3-3 beta protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human 14-3-3 beta
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-YWHAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-YWHAB antibody
Rabbit polyclonal antibody to 14-3-3 beta
Applications Tested/Suitable for anti-YWHAB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-YWHAB antibody
Western Blot: 1/500 - 1/2000, Immunohistochemistry: 1/50 - 1/200, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200

Western Blot (WB) of anti-YWHAB antibody
Western blot analysis of 14-3-3 beta expression in SW480 (A), HepG2 (B), mouse liver (C), mouse kidney (D) whole cell lysates.
anti-YWHAB antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-YWHAB antibody
Immunohistochemical analysis of 14-3-3 beta staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-YWHAB antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-YWHAB antibody
Immunofluorescent analysis of 14-3-3 beta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
anti-YWHAB antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for YWHAB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,850 Da
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
NCBI Official Symbol
YWHAB  [Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
  [Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
UniProt Gene Name
YWHAB  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433B_HUMAN
NCBI Summary for YWHAB
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for YWHAB
14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; perinuclear region of cytoplasm

Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; protein binding; protein domain specific binding

Biological Process: cytoplasmic sequestering of protein; MAPKKK cascade; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; regulation of mRNA stability
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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