• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-14-3-3 antibody :: Rabbit 14-3-3 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS440071
Unit / Price
Scan QR to view Datasheet
  0.2 mg  /  $240 +1 FREE 8GB USB
anti-14-3-3 antibody
Product Name

14-3-3, Polyclonal Antibody

Full Product Name

14-3-3 (N19) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, rat, human
Specificity
Mouse, rat, and human 14-3-3, broadly reactive among the 14-3-3 family (recognizes alpha, beta, theta, and zeta/delta)
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (lot specific)
Origin
14-3-3 (N19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminal domain of human 14-3-3.
Immunogen
Synthetic peptide mapping to the amino terminal domain of human 14-3-3.
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Other Notes
Small volumes of anti-14-3-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-14-3-3 antibody
14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).
Applications Tested/Suitable for anti-14-3-3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-14-3-3 antibody
Recommended western blotting starting dilution 1:400. Broadly reactive among the 14-3-3 family, will recognize alpha, beta, zeta (delta) and theta 14-3-3.
Protein Family
Product References and Citations for anti-14-3-3 antibody
1. Yaffe MB. 2002. How do 14-3-3 proteins work?- - Gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett. 513(1):53-57.2. Liu MY, Cai S, Espejo A, Bedford MT, Walker CL. 2002. 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation sites. Cancer Res. 62(22): 6475-6480.3. Tzivion G, Shen YH, Zhu J. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene 20(44):6331-6338.4. Fu H, Subramanian RR, Masters SC. 2000. 14-3-3 proteins: structure, function, and regulation. Annu Rev Pharmacol Toxicol. 40:617-647.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000