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anti-TNFAIP3 antibody :: Rabbit A20 Polyclonal Antibody

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Catalog # MBS820465
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

A20 (TNFAIP3), Polyclonal Antibody

Popular Item
Also Known As

Anti-A20 Antibody

Product Synonym Names
OTUD7C; Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20
Antibody/Peptide Pairs
A20 peptide (MBS823308) is used for blocking the activity of A20 antibody (MBS820465)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
790
OMIM
191163
3D Structure
ModBase 3D Structure for P21580
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of A20 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A20. The exact sequence is proprietary.
Preparation and Storage
Shipping:Blue Ice.
Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-TNFAIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TNFAIP3 antibody
Rabbit polyclonal antibody to A20
Applications Tested/Suitable for anti-TNFAIP3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-TNFAIP3 antibody
WB (1/500 - 1/1000)
IH (1/100 - 1/200)
IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-TNFAIP3 antibody
Western blot analysis of A20 expression in HeLa (A), Daudi (B), RAW264.7 (C) whole cell lysates
anti-TNFAIP3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TNFAIP3 antibody
Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-TNFAIP3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-TNFAIP3 antibody
Immunofluorescent analysis of A20 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-TNFAIP3 antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for TNFAIP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 3
NCBI Official Synonym Full Names
tumor necrosis factor, alpha-induced protein 3
NCBI Official Symbol
TNFAIP3  [Similar Products]
NCBI Official Synonym Symbols
A20; OTUD7C; TNFA1P2
  [Similar Products]
NCBI Protein Information
tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 3
UniProt Synonym Protein Names
OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20Cleaved into the following 2 chains:A20p50; A20p37
UniProt Gene Name
TNFAIP3  [Similar Products]
UniProt Synonym Gene Names
OTUD7C; TNF alpha-induced protein 3  [Similar Products]
UniProt Entry Name
TNAP3_HUMAN
NCBI Summary for TNFAIP3
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Comments for TNFAIP3
TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: centrosome; lysosome; cytoplasm; nucleus; cytosol

Molecular Function: ubiquitin binding; protein binding; protease binding; DNA binding; protein self-association; zinc ion binding; ubiquitin-specific protease activity; ubiquitin-protein ligase activity; kinase binding; ligase activity

Biological Process: negative regulation of toll-like receptor 5 signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; negative regulation of interleukin-6 production; inflammatory response; negative regulation of bone resorption; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of cyclin-dependent protein kinase activity; B-1 B cell homeostasis; negative regulation of B cell activation; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta production; regulation of germinal center formation; protein oligomerization; inhibition of NF-kappaB transcription factor; response to muramyl dipeptide; negative regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of protein catabolic process; innate immune response; negative regulation of protein ubiquitination; regulation of defense response to virus by host; negative regulation of interferon type I production
Precautions
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Disclaimer
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