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anti-ABAT antibody :: Rabbit ABAT Polyclonal Antibody

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Catalog # MBS129841
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

ABAT, Polyclonal Antibody

Popular Item
Full Product Name

ABAT Polyclonal Antibody

Product Synonym Names
GABAT; NPD009; GABA-AT
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 613163
3D Structure
ModBase 3D Structure for P80404
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human ABAT
Calculated Molecular Weight
56kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-ABAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABAT antibody
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Product Categories/Family for anti-ABAT antibody
Applications Tested/Suitable for anti-ABAT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ABAT antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-ABAT antibody
Western blot analysis of extracts of various cell lines, using ABAT antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-ABAT antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ABAT antibody
Immunohistochemistry of paraffin-embedded rat kidney using ABAT antibody at dilution of 1:100 (40x lens).
anti-ABAT antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ABAT antibody
Immunohistochemistry of paraffin-embedded human liver injury using ABAT antibody at dilution of 1:100 (40x lens).
anti-ABAT antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ABAT antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using ABAT antibody at dilution of 1:100 (40x lens).
anti-ABAT antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-ABAT antibody
Immunofluorescence analysis of A549 cells using ABAT antibody. Blue: DAPI for nuclear staining.
anti-ABAT antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ABAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
500
NCBI Official Full Name
4-aminobutyrate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
4-aminobutyrate aminotransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
GABAT; NPD009; GABA-AT
  [Similar Products]
NCBI Protein Information
4-aminobutyrate aminotransferase, mitochondrial; GABA transferase; GABA transaminase; GABA aminotransferase; 4-aminobutyrate transaminase; gamma-amino-N-butyrate transaminase; (S)-3-amino-2-methylpropionate transaminase
UniProt Protein Name
4-aminobutyrate aminotransferase, mitochondrial
UniProt Synonym Protein Names
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22); GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT
UniProt Gene Name
UniProt Synonym Gene Names
GABAT; GABA-AT; GABA transaminase; GABA-T  [Similar Products]
UniProt Entry Name
GABT_HUMAN
NCBI Summary for ABAT
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ABAT
ABAT: Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. Defects in ABAT are a cause of GABA transaminase deficiency (GABATD). The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Carbohydrate Metabolism - butanoate; EC 2.6.1.19; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - propanoate; Transferase; Other Amino Acids Metabolism - beta-alanine; EC 2.6.1.22

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: neuron projection; mitochondrion; mitochondrial matrix; 4-aminobutyrate transaminase complex

Molecular Function: protein homodimerization activity; succinate-semialdehyde dehydrogenase binding; (S)-3-amino-2-methylpropionate transaminase activity; 4-aminobutyrate transaminase activity; pyridoxal phosphate binding

Biological Process: response to nicotine; copulation; response to drug; synaptic transmission; neurotransmitter catabolic process; response to ethanol; behavioral response to cocaine; neurotransmitter secretion; response to hypoxia; locomotory behavior; negative regulation of blood pressure; gamma-aminobutyric acid catabolic process; response to iron ion

Disease: Gaba-transaminase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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