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anti-ABCA1 antibody :: Rabbit ABCA1 Polyclonal Antibody

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Catalog # MBS176404
Unit / Price DISCONTINUED
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Western Blot (WB)
Product Name

ABCA1, Polyclonal Antibody

Also Known As

Anti-ABCA1 antibody

Product Synonym Names
ATP-binding cassette sub-family A member 1; ATP-binding cassette, sub-family A(ABC1), member 1; ABC 1 antibody; ABC Transporter 1 antibody; ABC-1 antibody; ABC1 antibody; ABCA 1 antibody; ABCA1 antibody; ABCA1_HUMAN antibody; ATP binding Cassette 1 antibody; ATP binding cassette sub family A ABC1 member 1 antibody; ATP binding cassette sub family A member 1 antibody; ATP binding cassette sub-family A member 1 antibody; ATP binding Cassette Transporter 1 antibody; ATP-binding Cassette 1 antibody; ATP-binding cassette sub-family A member 1 antibody; ATP-binding Cassette Transporter 1 antibody; CERP antibody; Cholesterol Efflux Regulatory Protein antibody; FLJ14958 antibody; HDLDT1 antibody; Membrane bound antibody; MGC164864 antibody; MGC165011 antibody; TD antibody; TGD antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
2261
OMIM
gene+604091
3D Structure
ModBase 3D Structure for O95477
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1 (2219-2236aa NFAKDQSDDDHLKDLSLH), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ABCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABCA1 antibody
Description: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family A member 1(ABCA1) detection. Tested with WB in Human, Mouse, Rat.
Background: ABCA1(ATP-binding cassette, sub-family A(ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein(CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux  pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.
Applications Tested/Suitable for anti-ABCA1 antibody
Western Blot (WB)

Western Blot (WB) of anti-ABCA1 antibody
Anti-ABCA1 antibody, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: HELA Cell Lysate
anti-ABCA1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ABCA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
254,302 Da
NCBI Official Full Name
ATP-binding cassette sub-family A member 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol
ABCA1  [Similar Products]
NCBI Official Synonym Symbols
TGD; ABC1; CERP; ABC-1; HDLDT1
  [Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family A member 1; membrane-bound; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein
UniProt Protein Name
ATP-binding cassette sub-family A member 1
UniProt Synonym Protein Names
ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein
Protein Family
UniProt Gene Name
ABCA1  [Similar Products]
UniProt Synonym Gene Names
ABC1; CERP; ABC-1; ATP-binding cassette 1  [Similar Products]
UniProt Entry Name
ABCA1_HUMAN
NCBI Summary for ABCA1
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for ABCA1
ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Transporter; Vesicle; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to plasma membrane; endocytic vesicle; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane

Molecular Function: apolipoprotein A-I receptor activity; ATPase activity; cholesterol binding; apolipoprotein binding; ATPase binding; small GTPase binding; apolipoprotein A-I binding; protein binding; syntaxin binding; phospholipid transporter activity; cholesterol transporter activity; anion transmembrane transporter activity; phospholipid binding; ATP binding; receptor binding

Biological Process: response to drug; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; intracellular cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cellular lipid metabolic process; endosome transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; platelet dense granule organization and biogenesis; lysosome organization and biogenesis; response to low density lipoprotein stimulus; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; phagocytosis, engulfment; response to nutrient; phospholipid homeostasis; peptide secretion

Disease: Hypoalphalipoproteinemia, Primary; Tangier Disease; Hypercholesterolemia, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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