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anti-ABCC4 antibody :: Goat ABCC4/MRP4 Polyclonal Antibody

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Catalog # MBS421051
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

ABCC4/MRP4, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-ABCC4/MRP4 Antibody

Product Synonym Names
Goat Anti-ABCC4 (aa70-82) Antibody; ABCC4; MRP4; MOATB; MOAT-B; EST170205; ATP-binding cassette, sub-family C (CFTR/MRP), member 4; canalicular multispecific organic anion transporter (ABC superfamily); Multidrug Resistance Protein 4; RP11-74A12.1; ATP-binding cassette, sub-family C, member; ABCC4 antibody; MRP4 antibody; MOATB antibody; MOAT-B antibody; EST170205 antibody; ATP-binding cassette; sub-family C (CFTR/MRP); member 4 antibody; canalicular multispecific organic anion transporter (ABC superfamily) antibody; Multidrug Resistance Protein 4 antibody; RP11-74A12.1 antibody; ATP-binding cassette; sub-family C; member 4 antibody; bA464I2.1 (ATP-binding cassette; sub-family C (CFTR/MRP); member 4) antibody; canalicular multispecific organic anion transporter (ABC superfamily) antibody; multidrug resistance-associated protein 4; multispecific organic anion transporter B antibody; ABCC4 / MRP4
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1325
Immunogen Sequence
CNGQPSTLTI FETAL
OMIM
605250
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ABCC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ABCC4 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ABCC4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data obtained from a previous batch and kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence: This antibody (from a previous batch) has been successfully used in ICC/IF for human primary hepatocytes as described in the following paper: Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G126-35, PMID: 20395535.

Immunohistochemistry (IHC) of anti-ABCC4 antibody
(4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-ABCC4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ABCC4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
88,138 Da
NCBI Official Full Name
multidrug resistance-associated protein 4 isoform 1
NCBI Official Synonym Full Names
ATP binding cassette subfamily C member 4
NCBI Official Symbol
ABCC4  [Similar Products]
NCBI Official Synonym Symbols
MRP4; MOATB; MOAT-B
  [Similar Products]
NCBI Protein Information
multidrug resistance-associated protein 4
UniProt Protein Name
Multidrug resistance-associated protein 4
UniProt Synonym Protein Names
ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B; MOAT-B
Protein Family
UniProt Gene Name
ABCC4  [Similar Products]
UniProt Synonym Gene Names
MRP4; MOAT-B  [Similar Products]
UniProt Entry Name
MRP4_HUMAN
NCBI Summary for ABCC4
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
UniProt Comments for ABCC4
ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Transporter; Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: basolateral plasma membrane; integral to membrane; membrane; plasma membrane; platelet dense granule membrane

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; anion transmembrane-transporting ATPase activity; ATP binding; ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism

Biological Process: cilium biogenesis; platelet degranulation; prostaglandin secretion; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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