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anti-ABCF1 antibody :: Rabbit ABCF1 Polyclonal Antibody

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Catalog # MBS9605271
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

ABCF1, Polyclonal Antibody

Popular Item
Also Known As

ABCF1 Antibody

Product Synonym Names
ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette, sub family F (GCN20), member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
845
3D Structure
ModBase 3D Structure for Q8NE71
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
ABCF1 antibody detects endogenous levels of total ABCF1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus, Nucleoplasm. Nucleus Envelope.
Tissue Specificity
Ubiquitous.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ABCF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABCF1 antibody
Function: Isoform 2 is required for efficient Cap-and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Subunit Structure: Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.
Post-translational Modifications: Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.
Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Applications Tested/Suitable for anti-ABCF1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ABCF1 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-ABCF1 antibody
Western blot analysis of ABCF1 Antibody expression in mouse brain and mouse lung tissues lysates.
anti-ABCF1 antibody Western Blot (WB) (WB) image
Immunofluorescene (IF) of anti-ABCF1 antibody
MBS9605271 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-ABCF1 antibody Immunofluorescene (IF) image
NCBI/Uniprot data below describe general gene information for ABCF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 95 kDa
Predicted: 96 kDa
NCBI Official Full Name
ATP-binding cassette sub-family F member 1 isoform a
NCBI Official Synonym Full Names
ATP binding cassette subfamily F member 1
NCBI Official Symbol
ABCF1  [Similar Products]
NCBI Official Synonym Symbols
ABC27; ABC50
  [Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family F member 1
UniProt Protein Name
ATP-binding cassette sub-family F member 1
UniProt Synonym Protein Names
ATP-binding cassette 50; TNF-alpha-stimulated ABC protein
Protein Family
UniProt Gene Name
ABCF1  [Similar Products]
UniProt Synonym Gene Names
ABC50  [Similar Products]
NCBI Summary for ABCF1
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]
UniProt Comments for ABCF1
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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