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anti-ABI1 antibody :: Rabbit ABI1 Polyclonal Antibody

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Catalog # MBS9202894
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

ABI1, Polyclonal Antibody

Full Product Name

ABI1 Antibody (N-term)

Product Synonym Names
Abl interactor 1; Abelson interactor 1; Abi-1; Abl-binding protein 4; AblBP4; Eps8 SH3 domain-binding protein; Eps8-binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain-binding protein 1; e3B1; ABI1; SSH3BP1
Antibody/Peptide Pairs
ABI1 peptide (MBS9227690) is used for blocking the activity of ABI1 antibody (MBS9202894)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
81-108
OMIM
603050
3D Structure
ModBase 3D Structure for Q8IZP0
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Specificity
This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ABI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABI1 antibody
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.
Applications Tested/Suitable for anti-ABI1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-ABI1 antibody
WB~~1:1000

Western Blot (WB) of anti-ABI1 antibody
Western blot analysis of ABI1 Antibody (N-term) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.
anti-ABI1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ABI1 antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-ABI1 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ABI1 antibody
Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ABI1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunofluorescence (IF) of anti-ABI1 antibody
Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
anti-ABI1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ABI1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55081
NCBI Official Full Name
abl interactor 1 isoform b
NCBI Official Synonym Full Names
abl-interactor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
  [Similar Products]
NCBI Protein Information
abl interactor 1
UniProt Protein Name
Abl interactor 1
UniProt Synonym Protein Names
Abelson interactor 1; Abi-1; Abl-binding protein 4; AblBP4; Eps8 SH3 domain-binding protein; Eps8-binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain-binding protein 1; e3B1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SSH3BP1; Abi-1; AblBP4; Eps8-binding protein; Nap1BP  [Similar Products]
UniProt Entry Name
ABI1_HUMAN
NCBI Summary for ABI1
This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Sep 2011]
UniProt Comments for ABI1
Abi-1: an SH3-containing adapter protein that regulates c-Abl-mediated phosphorylation of Mena. Binds the SH3 domains of epidermal growth factor receptor kinase substrate (EPS8) and of spectrin. May play a role in fusion of macropinocytic vesicles. Associated with congenital acute monocytic leukemia.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: postsynaptic membrane; growth cone; cytoskeleton; endoplasmic reticulum; lamellipodium; postsynaptic density; intracellular; cytosol; nucleus; cell junction; filopodium

Molecular Function: protein binding; cytoskeletal protein binding; protein complex binding; protein tyrosine kinase activator activity

Biological Process: negative regulation of cell proliferation; somitogenesis; peptidyl-tyrosine phosphorylation; actin polymerization and/or depolymerization; innate immune response; vascular endothelial growth factor receptor signaling pathway; cell motility; transmembrane receptor protein tyrosine kinase signaling pathway
Product References and Citations for anti-ABI1 antibody
Wang,C., et.al., Mol. Cancer Res. 5 (10), 1031-1039 (2007)
Carabeo,R.A., et.al., Cell. Microbiol. 9 (9), 2278-2288 (2007)

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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