View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Rabbit Activation Transcription Factor 2 Polyclonal Antibody Catalog # MBS625339 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Activation Transcription Factor 2, Polyclonal Antibody Full Product Name Activation Transcription Factor 2, phosphorylated (Thr69/71) (ATF2, CREB P1) Product Synonym Names Anti -Activation Transcription Factor 2, phosphorylated (Thr69/71) (ATF2, CREB P1) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Isotype IgG Host Rabbit Species Reactivity Human, Mouse, Rat Specificity Detects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71. It does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71. Species Crossreactivity: Mouse and rat. Purity/Purification Affinity PurifiedPurified by Protein A affinity chromatography. Form/Format Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added. Immunogen Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr69 and Thr71 of human ATF-2. Preparation and Storage May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of anti-Activation Transcription Factor 2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Activation Transcription Factor 2 antibody The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). Product Categories/Family for anti-Activation Transcription Factor 2 antibody Antibodies; Abs to Transcription Factors Applications Tested/Suitable for anti-Activation Transcription Factor 2 antibody ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC) Application Notes for anti-Activation Transcription Factor 2 antibody Suitable for use in ELISA, Western Blotting and Immunohistochemistry (Paraffin sections).Dilution: Western Blotting: 1:1000Immunohistochemistry (Paraffin): 1:50 Western Blot (WB) of anti-Activation Transcription Factor 2 antibody Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or anisomycin-treated (lanes 3 and 4), and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using MBS625339. Immunohistochemistry (IHC) of anti-Activation Transcription Factor 2 antibody Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using MBS625339. Immunohistochemistry (IHC) of anti-Activation Transcription Factor 2 antibody Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin treated (right), using MBS625339. TOP Molecular Weight 70kDSee Control Antigen, A0855-42C1.[Similar Products] TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP