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anti-AES antibody :: Rabbit AES Polyclonal Antibody

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Catalog # MBS150968
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

AES, Polyclonal Antibody

Full Product Name

AES Antibody

Product Synonym Names
AES; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; GRG; Amino-terminal enhancer of split; Gp130-associated protein GAM; Amino enhancer of split; amino-terminal enhancer of split
Product Gene Name
Antibody/Peptide Pairs
AES peptide (MBS152200) is used for blocking the activity of AES antibody (MBS150968)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600188
3D Structure
ModBase 3D Structure for Q08117
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
AES Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES.
Buffer
AES Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
AES antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-AES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AES antibody
AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Applications Tested/Suitable for anti-AES antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-AES antibody
AES antibody can be used for the detection of AES by Western blot at 1 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-AES antibody
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
anti-AES antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-AES antibody
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μg/mL.
anti-AES antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-AES antibody
Immunofluorescence of AES in Hela cells with AES antibody at 20 μg/mL.
anti-AES antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for AES. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,091 Da
NCBI Official Full Name
amino-terminal enhancer of split isoform a
NCBI Official Synonym Full Names
amino-terminal enhancer of split
NCBI Official Symbol
NCBI Official Synonym Symbols
GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
  [Similar Products]
NCBI Protein Information
amino-terminal enhancer of split; groucho-related protein 5; gp130-associated protein GAM
UniProt Protein Name
Amino-terminal enhancer of split
UniProt Synonym Protein Names
Gp130-associated protein GAM; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GRG; GRG5; Amino enhancer of split  [Similar Products]
UniProt Entry Name
AES_HUMAN
NCBI Summary for AES
The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for AES
AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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