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anti-ACAN antibody :: Rabbit Aggrecan Polyclonal Antibody

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Catalog # MBS9608165
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Aggrecan (ACAN), Polyclonal Antibody

Popular Item
Full Product Name

Aggrecan Antibody

Product Synonym Names
ACAN; AGC 1; AGC1; AGCAN; Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan 1; Aggrecan core protein; Aggrecan proteoglycan; Aggrecan structural proteoglycan of cartilage; Aggrecan1; ATEGQV; Cartilage specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122; Chondroitin sulfate proteoglycan core protein 1; CSPG 1; CSPG1; CSPGCP; JSCATE; Large aggregating proteoglycan; mcspg; mgsk16; MSK 16; MSK16; SEDK
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P16112
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Aggrecan antibody detects endogenous levels of total Aggrecan
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Aggrecan
Subcellular Location
Secreted; Extracellular Matrix.
Tissue Specificity
Restricted to cartilages.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-ACAN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACAN antibody
Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Subunit Structure: Interacts with FBLN1 (By similarity). Interacts with COMP.
Post-translational Modifications: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
Similarity: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Belongs to the aggrecan/versican proteoglycan family.
Applications Tested/Suitable for anti-ACAN antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ACAN antibody
WB: 1:1000-3000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-ACAN antibody
Western blot analysis of Aggrecan using 293 whole cell lysates
anti-ACAN antibody Western Blot (WB) (WB) image
Immunofluorescene (IF) of anti-ACAN antibody
MBS9608165 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ACAN antibody Immunofluorescene (IF) image
NCBI/Uniprot data below describe general gene information for ACAN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 250 kDa
Predicted: 262 kDa
NCBI Official Full Name
aggrecan core protein isoform 1
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
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NCBI Protein Information
aggrecan core protein
UniProt Protein Name
Aggrecan core protein
UniProt Synonym Protein Names
Cartilage-specific proteoglycan core protein; CSPCP
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1  [Similar Products]
NCBI Summary for ACAN
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACAN
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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