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anti-Akt antibody :: Rabbit Akt Polyclonal Antibody

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Catalog # MBS9409993
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

Akt, Polyclonal Antibody

Popular Item
Also Known As

Akt (Phospho-Thr308) Antibody

Product Synonym Names
C-AKT; PKB; PKB-alpha; RAC; RAC-PK-alpha
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
480
OMIM
114480
3D Structure
ModBase 3D Structure for P31749
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt.
Modification
Phospho-Thr308
Target Name
Akt
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-Akt antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Akt antibody
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Product Categories/Family for anti-Akt antibody
Applications Tested/Suitable for anti-Akt antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Akt antibody
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western Blot (WB) of anti-Akt antibody
Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt(Phospho-Thr308) Antibody.
anti-Akt antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-Akt antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody (left) or the same antibody preincubated with blocking peptide #51055 (right).
anti-Akt antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-Akt antibody
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt(Phospho-Thr308) Antibody.
anti-Akt antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-Akt antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody.
anti-Akt antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-Akt antibody
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody.
anti-Akt antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for Akt. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60kd
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol
NCBI Official Synonym Symbols
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
  [Similar Products]
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PKB; RAC; PKB; PKB alpha  [Similar Products]
UniProt Entry Name
AKT1_HUMAN
NCBI Summary for Akt
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
UniProt Comments for Akt
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: nucleoplasm; microtubule cytoskeleton; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; regulation of myelination; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; regulation of cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; response to heat; T cell costimulation; positive regulation of fat cell differentiation; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; response to oxidative stress; regulation of nitric-oxide synthase activity; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; inflammatory response; cell differentiation; activated T cell apoptosis; aging; negative regulation of proteolysis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; cell proliferation; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of vasoconstriction; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; phosphorylation

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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