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anti-AMPK alpha antibody :: Rabbit AMPK alpha Polyclonal Antibody

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Catalog # MBS835324
Unit / Price
  0.05 mg  /  $310 +1 FREE 8GB USB
Product Name

AMPK alpha, Polyclonal Antibody

Popular Item
Also Known As

AMPK alpha antibody

Product Synonym Names
Polyclonal AMPK alpha; Anti-AMPK alpha; 5-AMP-activated protein kinase anibody; Ab 174/172
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
AMPK alpha antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Biological Significance
AMPK, a member of the SNF1 (sucrose nonfermentor) kinase family AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase.
Immunogen
AMPK alpha antibody was raised in Rabbit using the peptide sequence around aa 172~176/170~174 (L-R-T-S-C) derived from Human AMPKA1/AMPKA2 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-AMPK alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AMPK alpha antibody
Rabbit polyclonal AMPK alpha antibody
Applications Tested/Suitable for anti-AMPK alpha antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-AMPK alpha antibody
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000

Immunofluorescence (IF) of anti-AMPK alpha antibody
Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1/AMPKa2 antibody
anti-AMPK alpha antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-AMPK alpha antibody
Western Blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKa1/AMPKa2 antibody
anti-AMPK alpha antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AMPK alpha antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKa1/AMPKa2 antibody (left) or the same antibody preincubated with with blocking peptide (right)
anti-AMPK alpha antibody Immunohistochemistry (IHC) (IHC) image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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