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anti-ANGPT1 antibody :: Rabbit Angiopoietin 1 Polyclonal Antibody

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Catalog # MBS9601357
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Angiopoietin 1 (ANGPT1), Polyclonal Antibody

Popular Item
Also Known As

Angiopoietin 1 Antibody

Product Synonym Names
AGP 1; AGP1; AGPT; ANG 1; ANG; ANG-1; ANG1; Angiopoietin-1; Angiopoietin1; ANGP1_HUMAN; ANGPT 1; Angpt1; KIAA0003; OTTHUMP00000227592; OTTHUMP00000227599; OTTHUMP00000227600
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
498
3D Structure
ModBase 3D Structure for Q15389
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Angiopoietin 1 antibody detects endogenous levels of total Angiopoietin 1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Angiopoietin 1
Subcellular Location
Secreted.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ANGPT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ANGPT1 antibody
Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Subunit Structure: Homooligomer. Interacts with TEK/TIE2.
Post-translational Modifications: Glycosylated.
Applications Tested/Suitable for anti-ANGPT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ANGPT1 antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-ANGPT1 antibody
Western blot analysis of Angiopoietin 1 expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.
anti-ANGPT1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ANGPT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 57 kDa
Predicted: 58 kDa
NCBI Official Full Name
angiopoietin-1 isoform 1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1  [Similar Products]
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
  [Similar Products]
NCBI Protein Information
angiopoietin-1
UniProt Protein Name
Angiopoietin-1
Protein Family
UniProt Gene Name
ANGPT1  [Similar Products]
UniProt Synonym Gene Names
KIAA0003; ANG-1  [Similar Products]
NCBI Summary for ANGPT1
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
UniProt Comments for ANGPT1
Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Product References and Citations for anti-ANGPT1 antibody
Wu X, Hu Z, Yin Y, Li Y, Zhang T; Journal: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. [Dose and timing of normal saline resuscitation on endothelial glycocalyx in early septic shock].

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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