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anti-ANGPT2 antibody :: Angiopoietin 2 Polyclonal Antibody

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Catalog # MBS177699
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
anti-ANGPT2 antibody
Product Name

Angiopoietin 2 (ANGPT2), Polyclonal Antibody

Popular Item
Also Known As

Anti-Angiopoietin 2 Antibody

Product Synonym Names
Angiopoietin-2; AGPT 2; Agpt2; ANG 2; ANG-2; ANG2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin-2; Angiopoietin2; ANGP2_HUMAN; ANGPT 2; ANGPT2; ANGPT2; Tie2 ligand; angiopoietin 2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
495
OMIM
601922
3D Structure
ModBase 3D Structure for O15123
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
E Coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ANGPT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ANGPT2 antibody
Description: Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with IHC-P in Human;Mouse;Rat.

Background: ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Applications Tested/Suitable for anti-ANGPT2 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ANGPT2 antibody
Immunohistochemistry:
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Ms, Rat
Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
By Heat: Boiling paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
NCBI/Uniprot data below describe general gene information for ANGPT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,848 Da[Similar Products]
NCBI Official Full Name
angiopoietin-2 isoform b
NCBI Official Synonym Full Names
angiopoietin 2
NCBI Official Symbol
ANGPT2  [Similar Products]
NCBI Official Synonym Symbols
ANG2; AGPT2
  [Similar Products]
NCBI Protein Information
angiopoietin-2
UniProt Protein Name
Angiopoietin-2
Protein Family
UniProt Gene Name
ANGPT2  [Similar Products]
UniProt Synonym Gene Names
ANG-2  [Similar Products]
UniProt Entry Name
ANGP2_HUMAN
NCBI Summary for ANGPT2
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ANGPT2
ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cell projection; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: metal ion binding; protein binding; receptor binding; receptor tyrosine kinase binding

Biological Process: angiogenesis; germ cell development; leukocyte migration; maternal process involved in pregnancy; negative regulation of angiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of positive chemotaxis; organ regeneration; positive regulation of angiogenesis; response to activity; response to glucose stimulus; response to hypoxia; response to mechanical stimulus; response to organic cyclic substance; response to radiation; signal transduction; Tie receptor signaling pathway
Product References and Citations for anti-ANGPT2 antibody
1. Bhandari, V., Choo-Wing, R., Lee, C. G., Zhu, Z., Nedrelow, J. H., Chupp, G. L., Zhang, X., Matthay, M. A., Ware, L. B., Homer, R. J., Lee, P. J., Geick, A., de Fougerolles, A. R., Elias, J. A. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death. Nature Med. 12: 1286-1293, 2006. 2. Kim, I., Kim, J.-H., Ryu, Y. S., Jung, S. H., Nah, J. J., Koh, G. Y. Characterization and expression of a novel alternatively spliced human angiopoietin-2. J. Biol. Chem. 275: 18550-18556, 2000. 3. Cheung AH, Stewart RJ, Marsden PA (Jun 1998). "Endothelial Tie2/Tek ligands angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23". Genomics 48 (3): 389-91. 4. Fiedler, Ulrike; Krissl Tanja, Koidl Stefanie, Weiss Cornelia, Koblizek Thomas, Deutsch Urban, Martiny-Baron Georg, Marmé Dieter, Augustin Hellmut G (Jan 2003). "Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats". J. Biol. Chem. (United States) 278 (3): 1721-7.

Precautions
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