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anti-ANAPC5 antibody :: Rabbit APC5 Polyclonal Antibody

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Catalog # MBS151299
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

APC5 (ANAPC5), Polyclonal Antibody

Also Known As

APC5 Antibody

Product Synonym Names
APC5; APC5; APC5; Anaphase-promoting complex subunit 5; Cyclosome subunit 5; anaphase promoting complex subunit 5
Antibody/Peptide Pairs
APC5 peptide (MBS153011) is used for blocking the activity of APC5 antibody (MBS151299)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
755
OMIM
606948
3D Structure
ModBase 3D Structure for Q9UJX4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
APC5 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.
Buffer
APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
APC5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-ANAPC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ANAPC5 antibody
APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
Applications Tested/Suitable for anti-ANAPC5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ANAPC5 antibody
APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-ANAPC5 antibody
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.
anti-ANAPC5 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ANAPC5 antibody
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.
anti-ANAPC5 antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-ANAPC5 antibody
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.
anti-ANAPC5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ANAPC5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,526 Da
NCBI Official Full Name
Anaphase-promoting complex subunit 5
NCBI Official Synonym Full Names
anaphase promoting complex subunit 5
NCBI Official Symbol
ANAPC5  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
anaphase-promoting complex subunit 5; cyclosome subunit 5
UniProt Protein Name
Anaphase-promoting complex subunit 5
UniProt Synonym Protein Names
Cyclosome subunit 5
UniProt Gene Name
ANAPC5  [Similar Products]
UniProt Synonym Gene Names
APC5; APC5  [Similar Products]
UniProt Entry Name
APC5_HUMAN
NCBI Summary for ANAPC5
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]
UniProt Comments for ANAPC5
APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol

Molecular Function: protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-ANAPC5 antibody
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 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
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 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
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