• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-APPL1 antibody :: Rabbit APPL Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS175536
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

APPL (APPL1), Polyclonal Antibody

Also Known As

Anti-APPL antibody

Product Synonym Names
DCC-interacting protein 13-alpha(Dip13-alpha); adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; Adapter protein containing PH domain antibody; Adaptor Protein Containing pH Domain antibody; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody; Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody; AKT2 INTERACTOR antibody; APPL 1 antibody; APPL1 antibody; DCC interacting protein 13 alpha antibody; DCC-interacting protein 13-alpha antibody; DIP 13 alpha antibody; DIP13 alpha antibody; Dip13-alpha antibody; DIP13alpha antibody; DP13A_HUMAN antibody; KIAA1428 antibody; PTB domain and leucine zipper motif 1 antibody; PTB Domain and Leucine Zipper Motif antibody; Signaling adaptor protein DIP13alpha antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
709
OMIM
604299
3D Structure
ModBase 3D Structure for Q9UKG1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human APPL (138-158aa NDHDAAINRYSRLSKKRENDK), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-APPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APPL1 antibody
Description: Rabbit IgG polyclonal antibody for DCC-interacting protein 13-alpha(APPL1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: DCC-interacting protein 13-alpha(APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.
Applications Tested/Suitable for anti-APPL1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB) of anti-APPL1 antibody
Anti-APPL antibody, MBS175536, Western blotting
WB: HELA Cell Lysate
anti-APPL1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for APPL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
79,663 Da
NCBI Official Full Name
DCC-interacting protein 13-alpha
NCBI Official Synonym Full Names
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
NCBI Official Symbol
APPL1  [Similar Products]
NCBI Official Synonym Symbols
APPL; DIP13alpha
  [Similar Products]
NCBI Protein Information
DCC-interacting protein 13-alpha; dip13-alpha; AKT2 interactor; signaling adaptor protein DIP13alpha; adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
UniProt Protein Name
DCC-interacting protein 13-alpha
UniProt Synonym Protein Names
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
Protein Family
UniProt Gene Name
APPL1  [Similar Products]
UniProt Synonym Gene Names
; Dip13-alpha  [Similar Products]
UniProt Entry Name
DP13A_HUMAN
NCBI Summary for APPL1
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]
UniProt Comments for APPL1
APPL: an effector protein of the small GTPase Rab5, a key regulator of endocytosis. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Contains a PH domain and an N-terminal PID domain. Its interaction with RAB5A/Rab5 is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Phosphorylated upon DNA damage.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: early endosome membrane; cytoplasm; endosome membrane; NuRD complex; vesicle membrane; nucleus; cytosol

Molecular Function: identical protein binding; protein kinase B binding; protein binding

Biological Process: cell proliferation; positive regulation of apoptosis; insulin receptor signaling pathway; regulation of glucose import; signal transduction; cell cycle

Disease: Maturity-onset Diabetes Of The Young, Type 14
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000