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anti-ARHGAP1 antibody :: Rabbit ARHGAP1 Polyclonal Antibody

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Catalog # MBS9602054
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

ARHGAP1, Polyclonal Antibody

Popular Item
Also Known As

ARHGAP1 Antibody

Product Synonym Names
ARHGAP 1; Arhgap1; CDC42 GTPase activating protein; Cdc42 GTPase-activating protein; CDC42GAP; GTPase activating protein rhoOGAP; GTPase-activating protein rhoOGAP; GTPase-activating protein, Rho, 1; OTTHUMP00000233485; OTTHUMP00000233486; p50 RhoGAP; p50-RhoGAP; p50rhoGAP; RHG01_HUMAN; Rho GTPase activating protein 1; Rho GTPase-activating protein 1; Rho related small GTPase protein activator; Rho type GTPase activating protein 1; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; RHOGAP; RHOGAP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
439
3D Structure
ModBase 3D Structure for Q07960
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
ARHGAP1 antibody detects endogenous levels of total ARHGAP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human ARHGAP1
Subcellular Location
Cytoplasm.
Tissue Specificity
Ubiquitous.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ARHGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARHGAP1 antibody
Function: GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Subunit Structure: Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.
Applications Tested/Suitable for anti-ARHGAP1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ARHGAP1 antibody
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-ARHGAP1 antibody
MBS9602054 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-ARHGAP1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-ARHGAP1 antibody
Western blot analysis of ARHGAP1 using HeLa whole cell lysates
anti-ARHGAP1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ARHGAP1 antibody
MBS9602054 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ARHGAP1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ARHGAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 50 kDa
Predicted: 51 kDa
NCBI Official Full Name
rho GTPase-activating protein 1
NCBI Official Synonym Full Names
Rho GTPase activating protein 1
NCBI Official Symbol
ARHGAP1  [Similar Products]
NCBI Official Synonym Symbols
RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP
  [Similar Products]
NCBI Protein Information
rho GTPase-activating protein 1
UniProt Protein Name
Rho GTPase-activating protein 1
UniProt Synonym Protein Names
CDC42 GTPase-activating protein; GTPase-activating protein rhoGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAP
UniProt Gene Name
ARHGAP1  [Similar Products]
UniProt Synonym Gene Names
CDC42GAP; RHOGAP1  [Similar Products]
NCBI Summary for ARHGAP1
This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]
UniProt Comments for ARHGAP1
GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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