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anti-ATG5 antibody :: Rabbit ATG5 Polyclonal Antibody

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Catalog # MBS150643
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

ATG5, Polyclonal Antibody

Popular Item
Full Product Name

ATG5 Antibody

Product Synonym Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae)
Antibody/Peptide Pairs
ATG5 peptide (MBS153237) is used for blocking the activity of ATG5 antibody (MBS150643)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A9UGY9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
Purity/Purification
ATG5 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.
Buffer
ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ATG5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATG5 antibody
ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
Applications Tested/Suitable for anti-ATG5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATG5 antibody
ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-ATG5 antibody
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 μg/mL.
anti-ATG5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ATG5 antibody
Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 2.5 μg/mL.
anti-ATG5 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-ATG5 antibody
Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 20 μg/mL.
anti-ATG5 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
32,447 Da
NCBI Official Full Name
ATG5 autophagy related 5 homolog (S. cerevisiae), isoform CRA_b
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
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NCBI Protein Information
autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name
Autophagy protein 5
Protein Family
UniProt Gene Name
UniProt Entry Name
A9UGY9_HUMAN
UniProt Comments for ATG5
ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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