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anti-B-actin antibody :: Rabbit B-actin Polyclonal Antibody

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Catalog # MBS668048
Unit / Price
  0.025 mg  /  $140 +1 FREE 8GB USB
  0.1 mg  /  $270 +1 FREE 8GB USB
Western Blot (WB)
Product Name

B-actin, Polyclonal Antibody

Also Known As

B-actin Polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
376
OMIM
162091
3D Structure
ModBase 3D Structure for Q12824
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse (Predicted Reactivity: Rat
Form/Format
Purified (Liquid)
Concentration
0.5mg/ml, 200µl (lot specific)
Antigen
B-actin
Immunogen
A partial recombinant B-actin protein (amino acids 1-200) was used as the immunogen for this antibody.
Preparation and Storage
Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.
Other Notes
Small volumes of anti-B-actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-B-actin antibody
Applications Tested/Suitable for anti-B-actin antibody
Western Blot (WB)
Application Notes for anti-B-actin antibody
Western Blot: 4-6 µg/ml

Western Blot (WB) of anti-B-actin antibody
Expression analysis of  B-actin. Anti- B-actin antibody (11-13012) was used at 4 µg/ml on (1) Raw, (2) 3T3, (3) Hela and (4) U87 lysates.
anti-B-actin antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for B-actin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,158 Da
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 isoform b
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
NCBI Official Symbol
SMARCB1  [Similar Products]
NCBI Official Synonym Symbols
RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
  [Similar Products]
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47; SNF5 homolog; SWI/SNF-related matrix-associated protein; hSNF5; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; protein phosphatase 1, regulatory subunit 144; sucrose nonfermenting, yeast, homolog-like 1
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
UniProt Synonym Protein Names
BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog; hSNF5
UniProt Gene Name
SMARCB1  [Similar Products]
UniProt Synonym Gene Names
BAF47; INI1; SNF5L1; BAF47; hSNF5  [Similar Products]
UniProt Entry Name
SNF5_HUMAN
NCBI Summary for B-actin
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for B-actin
SMARCB1: a core component of the BAF (hSWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Binds to double-stranded DNA. Interacts with EphB4 and MAEL. Interacts with GADD34. Binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity. Interacts with human papillomavirus 18 E1 protein to stimulates its viral replication. Belongs to the SNF5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Cell cycle regulation; Tumor suppressor

Chromosomal Location of Human Ortholog: 22q11.23|22q11

Cellular Component: XY body; nucleoplasm; SWI/SNF complex; protein complex; nuclear chromatin; nucleolus; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; Tat protein binding; p53 binding; transcription coactivator activity

Biological Process: nervous system development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; DNA integration; DNA repair; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; nucleosome disassembly; blastocyst hatching; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; cell differentiation

Disease: Mental Retardation, Autosomal Dominant 15; Rhabdoid Tumor Predisposition Syndrome 1; Schwannomatosis 1
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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