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anti-ctnnb1 antibody :: Rabbit b-Catenin (Tyr-142) Polyclonal Antibody

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Catalog # MBS472004
Unit / Price
  0.1 mL  /  $380 +1 FREE 8GB USB
Product Name

b-Catenin (Tyr-142) (ctnnb1), Polyclonal Antibody

Popular Item
Full Product Name

Anti-beta-Catenin (Tyr-142), phospho-specific [gamma-Catenin (Tyr-133)]

Product Synonym Names
b-Catenin (Tyr-142); phospho-specific; Catenin beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_007127.5 (7435502..7458779, complement)
3D Structure
ModBase 3D Structure for Q7ZU14
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho- beta -Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta -Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate-treated cell lines, such as A431 and human endothelial cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Immunogen
Phospho-beta-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma-Catenin. These human sequences are highly conserved in rat and mouse beta-Catenins.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Other Notes
Small volumes of anti-ctnnb1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ctnnb1 antibody
beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C terminal armadillo repeat of beta-Catenin. This phosphorylation regulates beta-Catenin binding to E cadherin. Thus, sitespecific tyrosine phosphorylation of beta-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
Applications Tested/Suitable for anti-ctnnb1 antibody
ELISA, Western Blot
Application Notes for anti-ctnnb1 antibody
ELISA: 1:2000
Western Blot: 1:500
ICC: 1:100

Testing Data of anti-ctnnb1 antibody
anti-ctnnb1 antibody Testing Data image
Immunocytochemistry of anti-ctnnb1 antibody
anti-ctnnb1 antibody Immunocytochemistry (ICC) image
NCBI/Uniprot data below describe general gene information for ctnnb1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
85,520 Da[Similar Products]
NCBI Official Full Name
b-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1
NCBI Official Symbol
ctnnb1  [Similar Products]
NCBI Official Synonym Symbols
ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
  [Similar Products]
NCBI Protein Information
catenin beta-1; beta-catenin
UniProt Protein Name
Catenin (Cadherin-associated protein), beta 1
Protein Family
UniProt Gene Name
ctnnb1  [Similar Products]
UniProt Entry Name
Q7ZU14_DANRE
Product References and Citations for anti-ctnnb1 antibody
• Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.
• Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
• Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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