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anti-BAD antibody :: Rabbit BAD Polyclonal Antibody

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Catalog # MBS9404157
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

BAD, Polyclonal Antibody

Popular Item
Full Product Name

BAD (Ab-136) Antibody

Product Synonym Names
Bbc2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61337
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of total BAD protein.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around aa.134~138 (S-R-S-A-P) derived from Mouse BAD.
Target Name
BAD
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-BAD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BAD antibody
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Product Categories/Family for anti-BAD antibody
Applications Tested/Suitable for anti-BAD antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BAD antibody
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western Blot (WB) of anti-BAD antibody
Western blot analysis of extracts from HT29 and HepG2 cells using BAD(MBS9404157) Antibody.
anti-BAD antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-BAD antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(MBS9404157) Antibody (left) or the same antibody preincubated with blocking peptide(right).
anti-BAD antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for BAD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
23kd
NCBI Official Full Name
bcl2-associated agonist of cell death isoform 1
NCBI Official Synonym Full Names
BCL2-associated agonist of cell death
NCBI Official Symbol
NCBI Official Synonym Symbols
Bbc2; AI325008
  [Similar Products]
NCBI Protein Information
bcl2-associated agonist of cell death
UniProt Protein Name
Bcl2-associated agonist of cell death
UniProt Synonym Protein Names
Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death
UniProt Gene Name
UniProt Synonym Gene Names
Bbc6; BAD  [Similar Products]
UniProt Entry Name
BAD_MOUSE
UniProt Comments for BAD
BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein phosphatase binding; lipid binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: apoptosis; positive regulation of proteolysis; positive regulation of apoptosis; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; regulation of caspase activity; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of T cell differentiation; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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