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anti-SMARCE1 antibody :: Rabbit BAF57 Polyclonal Antibody

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Catalog # MBS8247663
Unit / Price
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  0.05 mL  /  $195 +1 FREE 8GB USB
  0.1 mL  /  $250 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

BAF57 (SMARCE1), Polyclonal Antibody

Popular Item
Full Product Name

Anti-BAF57 Antibody

Product Synonym Names
BAF57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; BAF57
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603111
3D Structure
ModBase 3D Structure for Q969G3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of BAF57 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human BAF57
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-SMARCE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SMARCE1 antibody
Rabbit polyclonal antibody to BAF57
Applications Tested/Suitable for anti-SMARCE1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-SMARCE1 antibody
Western Blot: 1/500 - 1/2000, Immunohistochemistry: 1/50 - 1/200, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200, Immunoprecipitation: 1/50 - 1/200, Chromatin Immunoprecipitation: 1/20 - 1/100

Western Blot (WB) of anti-SMARCE1 antibody
Western blot analysis of BAF57 expression in K562 (A), SHSY5Y (B), SKOV3 (C) whole cell lysates.
anti-SMARCE1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SMARCE1 antibody
Immunohistochemical analysis of BAF57 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-SMARCE1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-SMARCE1 antibody
Immunofluorescent analysis of BAF57 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
anti-SMARCE1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for SMARCE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,390 Da
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol
SMARCE1  [Similar Products]
NCBI Official Synonym Symbols
CSS5; BAF57
  [Similar Products]
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Synonym Protein Names
BRG1-associated factor 57; BAF57
UniProt Gene Name
SMARCE1  [Similar Products]
UniProt Synonym Gene Names
BAF57; BAF57  [Similar Products]
UniProt Entry Name
SMCE1_HUMAN
NCBI Summary for SMARCE1
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
UniProt Comments for SMARCE1
SMARCE1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCE1 may be a cause of Coffin-Siris syndrome, a highly variable disease characterized by mental retardation associated with a broad spectrum of different clinical features. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nuclear chromatin; nuclear chromosome; nucleoplasm; nucleus; protein complex; SWI/SNF complex; transcriptional repressor complex

Molecular Function: chromatin binding; ligand-dependent nuclear receptor binding; N-acetyltransferase activity; nucleosomal DNA binding; protein binding; protein N-terminus binding

Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; negative regulation of transcription, DNA-dependent; nucleosome disassembly

Disease: Coffin-siris Syndrome 5; Meningioma, Familial, Susceptibility To
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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