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anti-BAG4 antibody :: Rabbit BAG4 Polyclonal Antibody

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Catalog # MBS9606223
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

BAG4, Polyclonal Antibody

Popular Item
Also Known As

BAG4 Antibody

Product Synonym Names
BAG 4; BAG family molecular chaperone regulator 4; BAG-4; BAG4; BAG4_HUMAN; Bcl 2 associated athanogene 4; Bcl-2-associated athanogene 4; BCL2 associated athanogene 4; Silencer of death domains; SODD
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
421
3D Structure
ModBase 3D Structure for O95429
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
BAG4 antibody detects endogenous levels of total BAG4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm.
Tissue Specificity
Ubiquitous.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-BAG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BAG4 antibody
Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria.
Subunit Structure: Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to the death domain of TNFRSF1A in the absence of TNF and thereby prevents binding of adapter molecules such as TRADD or TRAF2. Binds to the death domain of TNFRSF12. Interacts with PRKN.
Applications Tested/Suitable for anti-BAG4 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-BAG4 antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-BAG4 antibody
MBS9606223 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-BAG4 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-BAG4 antibody
Western blot analysis of extracts from K562 cells, using BAG4 antibody.
anti-BAG4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-BAG4 antibody
MBS9606223 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-BAG4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for BAG4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 49 kD
Predicted: 50 kDa
NCBI Official Full Name
BAG family molecular chaperone regulator 4 isoform 2
NCBI Official Synonym Full Names
BCL2 associated athanogene 4
NCBI Official Symbol
NCBI Official Synonym Symbols
SODD; BAG-4
  [Similar Products]
NCBI Protein Information
BAG family molecular chaperone regulator 4
UniProt Protein Name
BAG family molecular chaperone regulator 4
UniProt Synonym Protein Names
Bcl-2-associated athanogene 4; Silencer of death domains
UniProt Gene Name
UniProt Synonym Gene Names
SODD; BAG-4  [Similar Products]
NCBI Summary for BAG4
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Comments for BAG4
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (). Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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