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anti-BDNF antibody :: Rabbit BDNF Polyclonal Antibody

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Catalog # MBS175418
Unit / Price
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  0.1 mg  /  $280 +1 FREE 8GB USB
Western Blot (WB)
Product Name

BDNF, Polyclonal Antibody

Popular Item
Full Product Name

Anti-BDNF antibody

Product Synonym Names
Brain-derived neurotrophic factor(BDNF); brain-derived neurotrophic factor; Abrineurin antibody; BDNF antibody; BDNF_HUMAN antibody; Brain Derived Neurotrophic Factor antibody; Brain-derived neurotrophic factor antibody; MGC34632 antibody; Neurotrophin antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
610269
3D Structure
ModBase 3D Structure for P23560
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with : Human
Predicted to work with: Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human BDNF (129-148aa HSDPARRGELSVCDSISEWV), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-BDNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BDNF antibody
Description: Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB in Human, Mouse, Rat.
Background: Brain derived neurotrophic factor(BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.
Applications Tested/Suitable for anti-BDNF antibody
Western Blot (WB)
Application Notes for anti-BDNF antibody
Western Blot (WB):
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat
Antigen Retrieval: -

Tested Species: In-house tested species with positive results
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-BDNF antibody
Anti-BDNF antibody, MBS175418, Western blotting
All lanes: Anti BDNF (MBS175418) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 37KD
anti-BDNF antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BDNF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,116 Da
NCBI Official Full Name
Brain-derived neurotrophic factor
NCBI Official Synonym Full Names
brain-derived neurotrophic factor
NCBI Official Symbol
NCBI Official Synonym Symbols
ANON2; BULN2
  [Similar Products]
NCBI Protein Information
brain-derived neurotrophic factor; abrineurin; neurotrophin
UniProt Protein Name
Brain-derived neurotrophic factor
UniProt Synonym Protein Names
Abrineurin
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
BDNF_HUMAN
NCBI Summary for BDNF
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
UniProt Comments for BDNF
BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: extracellular space; synaptic vesicle; mitochondrial crista; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasm; dendrite; extracellular region; terminal button; perikaryon

Molecular Function: growth factor activity; neurotrophin TRKB receptor binding

Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; regulation of neuron differentiation; axon extension; response to hormone stimulus; positive regulation of long-term neuronal synaptic plasticity; response to vitamin A; negative regulation of neuroblast proliferation; ureteric bud development; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; negative regulation of neuron apoptosis; response to electrical stimulus; negative regulation of synaptic transmission, GABAergic; inner ear development; response to food; nervous system development; chronic inflammatory response; response to light intensity; neuron recognition; regulation of short-term neuronal synaptic plasticity; learning; regulation of axon extension; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; axon target recognition; response to hyperoxia; glutamate secretion; response to hypoxia; response to fluoxetine; nerve development; response to activity; positive regulation of neuron differentiation; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; gamma-aminobutyric acid signaling pathway

Disease: Bulimia Nervosa, Susceptibility To, 1; Obsessive-compulsive Disorder; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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