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anti-BHLHA15 antibody :: Rabbit BHLHA15 Polyclonal Antibody

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Catalog # MBS153611
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

BHLHA15, Polyclonal Antibody

Popular Item
Also Known As

BHLHA15 Antibody

Product Synonym Names
BHLHA15; MIST1; BHLHB8; MIST1; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; bHLHa15; basic helix-loop-helix family, member a15
Antibody/Peptide Pairs
BHLHA15 peptide (MBS153989) is used for blocking the activity of BHLHA15 antibody (MBS153611)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
60-110
OMIM
608606
3D Structure
ModBase 3D Structure for Q7RTS1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
BHLHA15 antibody is human, mouse and rat reactive.
Purity/Purification
BHLHA15 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15.
Buffer
BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.
Positive Control
Rat Small Intestine Tissue Lysate: MBS151680
Human small Intestine Tissue Slide: MBS154284
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (71%)
Preparation and Storage
BHLHA15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Other Notes
Small volumes of anti-BHLHA15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BHLHA15 antibody
BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Applications Tested/Suitable for anti-BHLHA15 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BHLHA15 antibody
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

Western Blot (WB) of anti-BHLHA15 antibody
Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide
anti-BHLHA15 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-BHLHA15 antibody
Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.
anti-BHLHA15 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence of anti-BHLHA15 antibody
Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 ug/mL.
anti-BHLHA15 antibody Immunofluorescence image
NCBI/Uniprot data below describe general gene information for BHLHA15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Predicted: 21 kDa

Observed: 24 kDa
NCBI Official Full Name
class A basic helix-loop-helix protein 15
NCBI Official Synonym Full Names
basic helix-loop-helix family, member a15
NCBI Official Symbol
BHLHA15  [Similar Products]
NCBI Official Synonym Symbols
MIST1; BHLHB8
  [Similar Products]
NCBI Protein Information
class A basic helix-loop-helix protein 15; MIST-1; class II bHLH protein MIST1; class B basic helix-loop-helix protein 8; muscle, intestine and stomach expression 1; basic helix-loop-helix domain containing, class B, 8
UniProt Protein Name
Class A basic helix-loop-helix protein 15
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 8; bHLHb8; Muscle, intestine and stomach expression 1; MIST-1
UniProt Gene Name
BHLHA15  [Similar Products]
UniProt Synonym Gene Names
BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1  [Similar Products]
UniProt Entry Name
BHA15_HUMAN
UniProt Comments for BHLHA15
MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3'.

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity

Biological Process: G-protein coupled receptor protein signaling pathway; cellular response to glucose starvation; transcription, DNA-dependent; cell-cell signaling; unfolded protein response; cell maturation; intracellular distribution of mitochondria; calcium-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; Golgi organization and biogenesis; mitochondrial calcium ion transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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