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anti-BPNT1 antibody :: Goat Bisphosphate 3'-nucleotidase Polyclonal Antibody

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Catalog # MBS420859
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Bisphosphate 3'-nucleotidase (BPNT1), Polyclonal Antibody

Full Product Name

Goat anti-Bisphosphate 3'-nucleotidase Antibody

Product Synonym Names
bisphosphate 3'-nucleotidase; 3'(2'), 5'-bisphosphate nucleotidase 1; PIP; BPntase; PAP-inositol-1,4-phosphatase; bisphosphate 3\1-nucleotidase antibody; 3\1(2\1); 5\1-bisphosphate nucleotidase 1 antibody; PIP antibody; BPntase antibody; PAP-inositol-1; 4-phosphatase antibody; Bisphosphate 3'-nucleotidase; Bisphosphate 3'-nucleotidase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
RNYDYYASRV PESIK
OMIM
604053
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-RNYDYYASRVPESIK, from the C Terminus of the protein sequence according to NP_006076.4.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-BPNT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BPNT1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-BPNT1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 33.4kDa according to NP_006076.4). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Spiegelberg et al, J Biol Chem.1999 May 7;274(19): 13619-28.; PMID: 10224133). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-BPNT1 antibody
(1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-BPNT1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BPNT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,189 Da
NCBI Official Full Name
3'(2'),5'-bisphosphate nucleotidase 1 isoform 1
NCBI Official Synonym Full Names
3'(2'), 5'-bisphosphate nucleotidase 1
NCBI Official Symbol
BPNT1  [Similar Products]
NCBI Official Synonym Symbols
PIP; HEL20
  [Similar Products]
NCBI Protein Information
3'(2'),5'-bisphosphate nucleotidase 1
UniProt Protein Name
3'(2'),5'-bisphosphate nucleotidase 1
UniProt Synonym Protein Names
Bisphosphate 3'-nucleotidase 1; PAP-inositol 1,4-phosphatase; PIP
UniProt Gene Name
BPNT1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
BPNT1_HUMAN
NCBI Summary for BPNT1
BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]
UniProt Comments for BPNT1
BPNT1: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4- trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.7; Energy Metabolism - sulfur; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; cytosol

Molecular Function: 3'(2'),5'-bisphosphate nucleotidase activity; inositol-1,4-bisphosphate 1-phosphatase activity; magnesium ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; dephosphorylation; nervous system development; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; phosphoinositide phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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