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anti-BRCC3 antibody :: Rabbit BRCC3 / BRCC36 Polyclonal Antibody

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Catalog # MBS241255
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

BRCC3 / BRCC36, Polyclonal Antibody

Also Known As

Rabbit Polyclonal (IgG) to Human BRCC3 / BRCC36

Product Synonym Names
Anti-BRCC3 / BRCC36 Antibody (N-Terminus) IHC-plus; BRCC3; C6.1A; BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; CXorf53; RP11-143H17.2; Human BRCC3; BRCC36
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
316
OMIM
300617
3D Structure
ModBase 3D Structure for P46736
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
An 18 amino acid peptide from near the amino terminus of humAn BRCC36
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
A 18 amino acid peptide from near the amino terminus of human BRCC36.
Immunogen Type
Synthetic peptide
Immunogen
BRCC3 / BRCC36 antibody was raised against a 18 amino acid peptide from near the amino terminus of human BRCC36.
Antigen Modification
N-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-BRCC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BRCC3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-BRCC3 antibody
IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-BRCC3 antibody
Anti-BRCC3 / BRCC36 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-BRCC3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-BRCC3 antibody
Anti-BRCC3 / BRCC36 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-BRCC3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-BRCC3 antibody
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/ml.
anti-BRCC3 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-BRCC3 antibody
Western blot of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
anti-BRCC3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BRCC3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,027 Da
NCBI Official Full Name
lys-63-specific deubiquitinase BRCC36 isoform 1
NCBI Official Synonym Full Names
BRCA1/BRCA2-containing complex, subunit 3
NCBI Official Symbol
BRCC3  [Similar Products]
NCBI Official Synonym Symbols
C6.1A; BRCC36; CXorf53
  [Similar Products]
NCBI Protein Information
lys-63-specific deubiquitinase BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36
UniProt Protein Name
Lys-63-specific deubiquitinase BRCC36
UniProt Synonym Protein Names
BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36
UniProt Gene Name
BRCC3  [Similar Products]
UniProt Synonym Gene Names
BRCC36; C6.1A; CXorf53  [Similar Products]
UniProt Entry Name
BRCC3_HUMAN
NCBI Summary for BRCC3
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]
UniProt Comments for BRCC3
BRCC3: Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 3.4.19.-; Ubiquitin-specific protease; Oncoprotein

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity

Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; proteolysis; G2/M transition DNA damage checkpoint; response to X-ray
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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