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anti-CALB1 antibody :: Rabbit Calbindin Polyclonal Antibody

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Catalog # MBS176994
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

Calbindin (CALB1), Polyclonal Antibody

Popular Item
Also Known As

Anti-Calbindin Antibody

Product Synonym Names
Calbindin; calbindin 1, 28kDa; avian-type antibody; CAB27 antibody; CALB 1 antibody; CALB antibody; CALB1 antibody; CALB1_HUMAN antibody; Calbindin 1 28kDa antibody; Calbindin antibody; Calbindin D28 antibody; D 28K antibody; D-28K antibody; D28K antibody; OTTHUMP00000166027 antibody; OTTHUMP00000225441 antibody; RTVL H protein antibody; Vitamin D dependent calcium binding protein antibody; Vitamin D dependent calcium binding protein avian type antibody; Vitamin D dependent calcium binding protein avian-type antibody; Vitamin D-dependent calcium-binding protein antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
261
OMIM
114050
3D Structure
ModBase 3D Structure for P05937
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
E Coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CALB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CALB1 antibody
Description: Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Applications Tested/Suitable for anti-CALB1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CALB1 antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Rat
Predicted Species: Hu
Antigen Retrieval: -

Immunohistochemistry (Paraffin-embedded Section) Concentration: 0.5-1 ug/ml
Tested Species: Hu, Ms, Rat
Predicted Species: -
Antigen Retrieval: By Heat

WB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions.
Tested Species: in-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Immunohistochemistry (IHC) of anti-CALB1 antibody
Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CALB1 antibody
Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CALB1 antibody
Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CALB1 antibody
Figure 4. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-CALB1 antibody
Figure 5. Western blot analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calbindin at approximately 37KD. The expected band size for Calbindin is at 30KD.
anti-CALB1 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-CALB1 antibody
Figure 6. Flow Cytometry analysis of SiHa cells using anti-Calbindin antibody (MBS176994).
Overlay histogram showing SiHa cells stained with MBS176994 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (MBS176994,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
anti-CALB1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Flow Cytometry (FC/FACS) of anti-CALB1 antibody
Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (MBS176994).
Overlay histogram showing Hela cells stained with MBS176994 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (MBS176994,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
anti-CALB1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for CALB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,609 Da
NCBI Official Full Name
Calbindin
NCBI Official Synonym Full Names
calbindin 1, 28kDa
NCBI Official Symbol
CALB1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
UniProt Protein Name
Calbindin
UniProt Synonym Protein Names
Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type
Protein Family
UniProt Gene Name
CALB1  [Similar Products]
UniProt Synonym Gene Names
CAB27  [Similar Products]
UniProt Entry Name
CALB1_HUMAN
NCBI Summary for CALB1
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]
UniProt Comments for CALB1
CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: cell soma; axon; dendrite; synapse; nucleus; cytosol

Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding

Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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