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anti-CASP3 antibody :: Rabbit Caspase-3 Polyclonal Antibody

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Catalog # MBS2535040
Unit / Price
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Product Name

Caspase-3 (CASP3), Polyclonal Antibody

Popular Item
Also Known As

Caspase-3 Polyclonal Antibody

Product Synonym Names
CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
277
OMIM
600636
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from the Internal region of human Caspase-3.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CASP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CASP3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CASP3 antibody
WB: 1:500-1:2000, ELISA: 1:5000-1:20000

Western Blot (WB) of anti-CASP3 antibody
Western Blot analysis of various cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.
anti-CASP3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CASP3 antibody
Immunohistochemistry of paraffin-embedded Rat kidney tissue using Caspase-3 Polyclonal Antibody at dilution of 1:200.
anti-CASP3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CASP3 antibody
Immunofluorescence analysis of Rat lung tissue using Caspase-3 Polyclonal Antibody at dilution of 1:200.
anti-CASP3 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CASP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Calculated MW: 32
Observed MW: 35[Similar Products]
NCBI Official Full Name
caspase-3 preproprotein
NCBI Official Synonym Full Names
caspase 3
NCBI Official Symbol
CASP3  [Similar Products]
NCBI Official Synonym Symbols
CPP32; SCA-1; CPP32B
  [Similar Products]
NCBI Protein Information
caspase-3
UniProt Protein Name
Caspase-3
UniProt Synonym Protein Names
Apopain; Cysteine protease CPP32; CPP-32
Protein Family
UniProt Gene Name
CASP3  [Similar Products]
UniProt Synonym Gene Names
CPP32; CASP-3; CPP-32; SCA-1  [Similar Products]
UniProt Entry Name
CASP3_HUMAN
NCBI Summary for CASP3
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Comments for CASP3
CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; Protease; Motility/polarity/chemotaxis; EC 3.4.22.56

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: cytosol; lipid raft; nucleoplasm; nucleus; plasma membrane

Molecular Function: cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; caspase activation via cytochrome c; cell fate commitment; cell structure disassembly during apoptosis; DNA fragmentation during apoptosis; erythrocyte differentiation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; hippocampus development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; platelet formation; positive regulation of neuron apoptosis; programmed cell death; proteolysis; regulation of caspase activity; response to amino acid stimulus; response to antibiotic; response to cobalt ion; response to DNA damage stimulus; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to lipopolysaccharide; response to nicotine; response to UV; response to X-ray; sensory perception of sound; T cell homeostasis; wound healing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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