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anti-CBX4 antibody :: Rabbit CBX4 Polyclonal Antibody

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Catalog # MBS150226
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

CBX4, Polyclonal Antibody

Also Known As

CBX4 Antibody

Product Synonym Names
CBX4; PC2; NBP16; E3 SUMO-protein ligase CBX4; Chromobox protein homolog 4; Pc2; chromobox homolog 4
Antibody/Peptide Pairs
CBX4 peptide (MBS152340) is used for blocking the activity of CBX4 antibody (MBS150226)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
560
OMIM
603079
3D Structure
ModBase 3D Structure for O00257
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Two alternatively spliced transcript variants have been observed.
Purity/Purification
CBX4 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4.
Buffer
CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
CBX4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-CBX4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CBX4 antibody
CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.
Applications Tested/Suitable for anti-CBX4 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-CBX4 antibody
CBX4 antibody can be used for detection of CBX4 by Western blot at 1 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-CBX4 antibody
Western blot analysis of CBX4 in human brain tissue lysate with CBX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
anti-CBX4 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-CBX4 antibody
Immunofluorescence of CBX4 in human brain tissue with CBX4 antibody at 20 μg/mL.
anti-CBX4 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CBX4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62 kDa
NCBI Official Full Name
E3 SUMO-protein ligase CBX4
NCBI Official Synonym Full Names
chromobox homolog 4
NCBI Official Symbol
NCBI Official Synonym Symbols
PC2; NBP16
  [Similar Products]
NCBI Protein Information
E3 SUMO-protein ligase CBX4; Pc class 2 homolog; polycomb 2 homolog; NS5ATP1-binding protein 16; chromobox protein homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila)
UniProt Protein Name
E3 SUMO-protein ligase CBX4
UniProt Synonym Protein Names
Chromobox protein homolog 4; Polycomb 2 homolog; Pc2; hPc2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Pc2; hPc2  [Similar Products]
UniProt Entry Name
CBX4_HUMAN
UniProt Comments for CBX4
CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; SUMO ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; nuclear speck; PcG protein complex; nucleus

Molecular Function: protein binding; enzyme binding; single-stranded RNA binding; SUMO binding; phosphoprotein binding; chromatin binding; transcription corepressor activity; methylated histone residue binding; ligase activity

Biological Process: protein sumoylation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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