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anti-Cd14 antibody :: Rabbit CD14 Polyclonal Antibody

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Catalog # MBS9216116
Unit / Price
  0.1 mL  /  $340 +1 FREE 8GB USB
Product Name

CD14, Polyclonal Antibody

Popular Item
Also Known As

Rabbit Anti-CD14 Polyclonal Antibody

Product Synonym Names
Purified Rabbit Polyclonal Antibody (Pab); Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
366
3D Structure
ModBase 3D Structure for P10810
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
Function
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:16148141). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135, PubMed:15895089). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (By similarity). Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity).
Cellular location
Cell membrane; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P08571}. Secreted {ECO:0000250|UniProtKB:P08571}. Membrane raft{ECO:0000250|UniProtKB:P08571}. Golgiapparatus{ECO:0000250|UniProtKB:P08571}.Note=Soluble, secreted forms seem to exist. They may arise by cleavage of the GPI anchor{ECO:0000250|UniProtKB:P08571
Tissue Location
Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712)
Preparation and Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01 PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C .
Other Notes
Small volumes of anti-Cd14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Cd14 antibody
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).
Applications Tested/Suitable for anti-Cd14 antibody
Western Blot(WB),Immunohistochemistry-Paraffin(IHC-P), Flow Cytometry (FC)
Application Notes for anti-Cd14 antibody
Western Blot (WB)~~1:100~1:500
Immunohistochemistry- Paraffin (IHC-P)~~1:100~1:500
Flow Cytometry (FC)~~1:20~1:100

Testing Data (TD) of anti-Cd14 antibody
Mouse lung lysates probed with Rabbit Anti-CD14 Polyclonal Antibody, Unconjugated (MBS9216116) at 1:300 overnight at 4 ?C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37 ?C.
anti-Cd14 antibody Testing Data (TD) image
Testing Data (TD) of anti-Cd14 antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (MBS9216116) at 1:200 followed by conjugation to the secondary antibody and DAB staining
anti-Cd14 antibody Testing Data (TD) image
Testing Data (TD) of anti-Cd14 antibody
A549 cells probed with C D14 Polyclonal Antibody, Unconjugated AP52111 at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue),secondary only (light blue) and isotype control (orange).
anti-Cd14 antibody Testing Data (TD) image
NCBI/Uniprot data below describe general gene information for Cd14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 antigen
NCBI Official Symbol
NCBI Protein Information
monocyte differentiation antigen CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
UniProt Gene Name
UniProt Entry Name
CD14_MOUSE
NCBI Summary for Cd14
This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]
UniProt Comments for Cd14
CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; extracellular space; cell surface; membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane

Molecular Function: lipopolysaccharide binding

Biological Process: response to bacterium; immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; positive regulation of tumor necrosis factor production; inflammatory response; positive regulation of cytokine secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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