• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CD4 antibody :: Rabbit CD4 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS551067
Unit / Price
  0.1 mg  /  $280 +1 FREE 8GB USB
  0.2 mg  /  $385 +1 FREE 8GB USB
Immunohistochemistry
Product Name

CD4, Polyclonal Antibody

Popular Item
Also Known As

Rabbit anti CD4 Polyclonal

Product Synonym Names
Rabbit anti CD4 Polyclonal purified; cd4; cd-4; polyclonal; PAb
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
207
OMIM
186830
3D Structure
ModBase 3D Structure for P07766
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Serum IgG fraction was purified by Protein G affinity chromatography.
Form/Format
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Source Note
Polyclonal CD4 antibody was produced from sera of rabbits immunized with synthetic protein containing CD4 antigenic determinants.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Other Notes
Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD4 antibody
CD4 is a 55 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. In humans CD4 is expressed of peripheral T-cells, thymocytes, on macrophages and granulocytes. CD4 acts as a cellular adhesion molecule binding MHC class 2 molecules. It stabilizes the interaction of MHC class 2 restricted T- cells and antigen-presenting cells expressing an antigen in combination with a MHC class 2 molecule. Interactions of CD4 with MHC class 2 molecules are crucial during thymic development and subsequently for the function of single- positive CD4 (+), CD8 (-) T- lymphocytes. CD4 functions as an effector molecule for intracellular signal transduction. In T- lymphocytes CD4 has been found to be associated with tyrosine kinase lck which phosphorylates a number of other cell surface proteins and is involved probably in transducing the intracellular signal when these proteins are bound to their receptors.
Product Categories/Family for anti-CD4 antibody
Applications Tested/Suitable for anti-CD4 antibody
Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
Application Notes for anti-CD4 antibody
ELISA: Use at a concentration range of 1-2ug/ml.
WB: A suitable range of concentrations of this antibody for WB is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of FFPE human colorectal cancer sections showing lymphocyte expression of CD4.
It was used as primary antibody, dilution 1:400, DAB chromogenic staining.

Immunohistochemical analysis of FFPE mouse kidney sections showing lymphocyte expression of CD4.
It was used as primary antibody, dilution 1:200, DAB chromogenic staining.

Immunohistochemistry of anti-CD4 antibody
anti-CD4 antibody Immunohistochemistry (IHC) image
NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,147 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names
T-cell surface antigen T3/Leu-4 epsilon chain
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CD3E_HUMAN
NCBI Summary for CD4
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
UniProt Comments for CD4
CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000