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anti-CDC25C antibody :: Rabbit CDC25C Polyclonal Antibody

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Catalog # MBS419762
Unit / Price
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  0.1 mg  /  $385 +1 FREE 8GB USB
Product Name

CDC25C, Polyclonal Antibody

Full Product Name

Anti-CDC25C Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M34065 mRNA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Western blot analysis of extracts of K562 cell lines, using CDC25C antibody. Immunohistochemistry of paraffin-embedded rat brain using CDC25C antibody at dilution of 1:100 (400x lens). Immunohistochemistry of paraffin-embedded rat kidney using CDC25C antibody at dilution of 1:100 (400x lens). Immunofluorescence analysis of U2OS cell using CDC25C antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant protein of human CDC25C
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-CDC25C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDC25C antibody
Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
Applications Tested/Suitable for anti-CDC25C antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CDC25C antibody
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
ICC: 1:50- 1:200
IP: 1:50- 1:100
IF: 1:50- 1:200

Western Blot (WB) of anti-CDC25C antibody
anti-CDC25C antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CDC25C antibody
anti-CDC25C antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CDC25C antibody
anti-CDC25C antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CDC25C antibody
anti-CDC25C antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CDC25C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,608 Da
NCBI Official Full Name
CDC25C, partial
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
CDC25C  [Similar Products]
NCBI Official Synonym Symbols
CDC25; PPP1R60
  [Similar Products]
NCBI Protein Information
M-phase inducer phosphatase 3
UniProt Protein Name
M-phase inducer phosphatase 3
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25C
UniProt Gene Name
CDC25C  [Similar Products]
NCBI Summary for CDC25C
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
UniProt Comments for CDC25C
CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: phosphoprotein phosphatase activity; protein binding; protein kinase binding; WW domain binding

Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA replication; G2/M transition of mitotic cell cycle; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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