• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CDK1 antibody :: Rabbit CDK1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS125315
Unit / Price
Scan QR to view Datasheet
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

CDK1, Polyclonal Antibody

Popular Item
Full Product Name

CDK1 Polyclonal Antibody

Product Synonym Names
CDC2; CDC28A; CDK1; DKFZp686L20222; MGC111195
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
116940
3D Structure
ModBase 3D Structure for P06493
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Immunogen
Center-peptide of human CDK1
Calculated Molecular Weight
34kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-CDK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDK1 antibody
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
Product Categories/Family for anti-CDK1 antibody
Applications Tested/Suitable for anti-CDK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CDK1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-CDK1 antibody
Western blot analysis of extracts of various cell lines, using CDK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
anti-CDK1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CDK1 antibody
Immunohistochemistry of paraffin-embedded rat brain using CDK1 antibody at dilution of 1:100 (40x lens).
anti-CDK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CDK1 antibody
Immunohistochemistry of paraffin-embedded mouse spinal cord using CDK1 antibody at dilution of 1:100 (40x lens).
anti-CDK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CDK1 antibody
Immunohistochemistry of paraffin-embedded mouse brain using CDK1 antibody at dilution of 1:100 (40x lens).
anti-CDK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CDK1 antibody
Immunofluorescence analysis of A549 cells using CDK1 antibody. Blue: DAPI for nuclear staining.
anti-CDK1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CDK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
297
NCBI Official Full Name
Cyclin-dependent kinase 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
CDC2; CDC28A; P34CDC2
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
UniProt Protein Name
Cyclin-dependent kinase 1
UniProt Synonym Protein Names
Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
UniProt Gene Name
UniProt Synonym Gene Names
CDC2; CDC28A; CDKN1; P34CDC2; CDK1  [Similar Products]
UniProt Entry Name
CDK1_HUMAN
NCBI Summary for CDK1
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for CDK1
CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.22; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 2.7.11.23; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; centrosome; mitochondrion; membrane; spindle microtubule; cytoplasm; midbody; cytosol; nucleus

Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: regulation of Schwann cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; response to toxin; histone phosphorylation; regulation of embryonic development; ventricular cardiac muscle cell development; centrosome cycle; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; toll-like receptor 9 signaling pathway; response to activity; negative regulation of apoptosis; response to amine stimulus; G1/S transition of mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; peptidyl-threonine phosphorylation; microtubule cytoskeleton organization and biogenesis; DNA repair; MyD88-dependent toll-like receptor signaling pathway; G1/S-specific transcription in mitotic cell cycle; peptidyl-serine phosphorylation; response to cadmium ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to copper ion; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000