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anti-CDK5RAP3 antibody :: Rabbit CDK5RAP3 Polyclonal Antibody

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Catalog # MBS9605004
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

CDK5RAP3, Polyclonal Antibody

Popular Item
Also Known As

CDK5RAP3 Antibody

Product Synonym Names
C53; CDK5 activator binding protein C53; CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein 3; CDK5 regulatory subunit-associated protein 3; Cdk5rap3; CK5P3_HUMAN; HSF 27; HSF27; IC53; Ischemic heart CDK5 activator binding protein C53; LXXLL/leucine zipper containing ARFbinding protein; LZAP; MST016; OK/SW cl.114; PP1553; Protein HSF-27
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
531
3D Structure
ModBase 3D Structure for Q96JB5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
CDK5RAP3 antibody detects endogenous levels of total CDK5RAP3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus; Cytoskeleton
Tissue Specificity
Ubiquitously expressed (PubMed:12054757, PubMed:10721722). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta (PubMed:12737517).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CDK5RAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDK5RAP3 antibody
Function: Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).
Subunit Structure: Interacts with CDK5R1; competes with CDK5RAP1 and CDK5RAP2 (PubMed:12737517). Interacts with RELA (PubMed:17785205). Interacts with CHEK1; may negatively regulate CHEK1 and thereby stimulate entry into mitosis (PubMed:19223857). Interacts with CDKN2A/ARF and MDM2; forms a ternary complex involved in regulation of p53/TP53 (PubMed:16173922). Interacts with UFL1; the interaction is direct (PubMed:20164180, PubMed:20228063). Interacts with DDRGK1 (PubMed:20228063). Interacts with MAPK14 (PubMed:21283629). Interacts with CCNB1 (PubMed:15790566). Interacts with TUBG1; may regulate CDK5RAP3 in mitotic G2/M transition checkpoint (PubMed:21465471).
Post-translational Modifications: May be phosphorylated by CDK5. Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1. May be ufmylated. Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.
Similarity: Belongs to the CDK5RAP3 family.
Applications Tested/Suitable for anti-CDK5RAP3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CDK5RAP3 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-CDK5RAP3 antibody
MBS9605004 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-CDK5RAP3 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-CDK5RAP3 antibody
Western blot analysis of extracts from 293 cells, using CDK5RAP3 antibody.The lane on the left is treated with the antigen-specific peptide.
anti-CDK5RAP3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CDK5RAP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 60 kD
Predicted: 57 kDa
NCBI Official Full Name
CDK5 regulatory subunit-associated protein 3 isoform c
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 3
NCBI Official Symbol
CDK5RAP3  [Similar Products]
NCBI Official Synonym Symbols
C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114
  [Similar Products]
NCBI Protein Information
CDK5 regulatory subunit-associated protein 3
UniProt Protein Name
CDK5 regulatory subunit-associated protein 3
UniProt Synonym Protein Names
CDK5 activator-binding protein C53
Protein Family
UniProt Gene Name
CDK5RAP3  [Similar Products]
UniProt Synonym Gene Names
IC531 PublicationManual assertion based on opinion iniRef.15  [Similar Products]
NCBI Summary for CDK5RAP3
This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]
UniProt Comments for CDK5RAP3
Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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