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anti-CHST10 antibody :: Rabbit CHST10 Polyclonal Antibody

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Catalog # MBS9605337
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

CHST10, Polyclonal Antibody

Popular Item
Also Known As

CHST10 Antibody

Product Synonym Names
Carbohydrate sulfotransferase 10; CHST10; CHSTA_HUMAN; EC 2.8.2.-; HNK-1 sulfotransferase; HNK-1ST; HNK1ST; huHNK-1ST; MGC17148; OTTHUMP00000203188
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
356
3D Structure
ModBase 3D Structure for O43529
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
CHST10 antibody detects endogenous levels of total CHST10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CHST10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHST10 antibody
Function: Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein-and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.
Similarity: Belongs to the sulfotransferase 2 family.
Applications Tested/Suitable for anti-CHST10 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CHST10 antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-CHST10 antibody
MBS9605337 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-CHST10 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-CHST10 antibody
Western blot analysis of extracts from HuvEc cells, using CHST10 antibody.
anti-CHST10 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CHST10 antibody
MBS9605337 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-CHST10 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CHST10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 48 kD
Predicted: 43 kDa
NCBI Official Full Name
carbohydrate sulfotransferase 10
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 10
NCBI Official Symbol
CHST10  [Similar Products]
NCBI Official Synonym Symbols
HNK1ST; HNK-1ST
  [Similar Products]
NCBI Protein Information
carbohydrate sulfotransferase 10
UniProt Protein Name
Carbohydrate sulfotransferase 10
UniProt Synonym Protein Names
HNK-1 sulfotransferase; HNK-1ST; HNK1ST; HuHNK-1ST
UniProt Gene Name
CHST10  [Similar Products]
UniProt Synonym Gene Names
HNK-1ST; HNK1ST; HuHNK-1ST  [Similar Products]
NCBI Summary for CHST10
This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011]
UniProt Comments for CHST10
Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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