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anti-NOTCH2 antibody :: Rabbit Cleaved-Notch 2 Polyclonal Antibody

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Catalog # MBS9410510
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

Cleaved-Notch 2 (NOTCH2), Polyclonal Antibody

Also Known As

Cleaved-Notch 2 (D1733) Polyclonal Antibody

Product Synonym Names
NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1235
OMIM
102500
3D Structure
ModBase 3D Structure for Q04721
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Cleaved-Notch 2 (D1733) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to D1733.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the Internal region of human Notch 2.
Target Name
Cleaved-Notch 2
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-NOTCH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-NOTCH2 antibody
Applications Tested/Suitable for anti-NOTCH2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-NOTCH2 antibody
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:40000
Not yet tested in other applications.

Western Blot (WB) of anti-NOTCH2 antibody
Western Blot analysis of 293 cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody
anti-NOTCH2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NOTCH2 antibody
Western Blot analysis of KB cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody
anti-NOTCH2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NOTCH2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
265,405 Da[Similar Products]
NCBI Official Full Name
neurogenic locus notch homolog protein 2 isoform 2
NCBI Official Synonym Full Names
notch 2
NCBI Official Symbol
NOTCH2  [Similar Products]
NCBI Official Synonym Symbols
hN2; AGS2; HJCYS
  [Similar Products]
NCBI Protein Information
neurogenic locus notch homolog protein 2
UniProt Protein Name
Neurogenic locus notch homolog protein 2
UniProt Gene Name
NOTCH2  [Similar Products]
UniProt Synonym Gene Names
N2ICD  [Similar Products]
UniProt Entry Name
NOTC2_HUMAN
NCBI Summary for NOTCH2
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Comments for NOTCH2
Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13-p11

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; extracellular region; nucleus; receptor complex; cilium

Molecular Function: protein binding; receptor activity; calcium ion binding

Biological Process: apoptosis; positive regulation of apoptosis; multicellular organismal development; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; hemopoiesis; cell cycle arrest; cell growth; positive regulation of BMP signaling pathway; embryonic limb morphogenesis; transcription initiation from RNA polymerase II promoter; nervous system development; Notch signaling pathway; intracellular receptor-mediated signaling pathway; in utero embryonic development; stem cell maintenance; Notch receptor processing; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; gene expression; positive regulation of Ras protein signal transduction; determination of left/right symmetry; negative regulation of apoptosis

Disease: Hajdu-cheney Syndrome; Alagille Syndrome 2
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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