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anti-CYB5A antibody :: Rabbit CYB5A Polyclonal Antibody

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Catalog # MBS9607958
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

CYB5A, Polyclonal Antibody

Popular Item
Also Known As

CYB5A Antibody

Product Synonym Names
CYB 5; CYB 5A; CYB5; CYB5_HUMAN; Cyb5a; Cytochrome b 5; Cytochrome b5 (microsomal); Cytochrome b5; Cytochrome b5 type A (microsomal); Cytochrome b5 type A; MCB 5; MCB5; Microsomal cytochrome b5; Microsomal cytochrome b5 type A; Type 1 cyt b5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
124
3D Structure
ModBase 3D Structure for P00167
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
CYB5A antibody detects endogenous levels of total CYB5A
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human CYB5A
Subcellular Location
Cytoplasm and Endoplasmic Reticulum Membrane. Microsome Membrane.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CYB5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CYB5A antibody
Description: The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.
Function: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Similarity: Belongs to the cytochrome b5 family.
Applications Tested/Suitable for anti-CYB5A antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CYB5A antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-CYB5A antibody
MBS9607958 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-CYB5A antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-CYB5A antibody
MBS9607958 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-CYB5A antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-CYB5A antibody
Western blot analysis of A549 whole cell lysates, using CYB5A Antibody. The lane on the left is treated with the antigen-specific peptide.
anti-CYB5A antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CYB5A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 15 kDa
Predicted: 16 kDa
NCBI Official Full Name
cytochrome b5 isoform 3
NCBI Official Synonym Full Names
cytochrome b5 type A
NCBI Official Symbol
CYB5A  [Similar Products]
NCBI Official Synonym Symbols
CYB5; MCB5; METAG
  [Similar Products]
NCBI Protein Information
cytochrome b5
UniProt Protein Name
Cytochrome b5
UniProt Synonym Protein Names
Microsomal cytochrome b5 type A; MCB5
Protein Family
UniProt Gene Name
CYB5A  [Similar Products]
UniProt Synonym Gene Names
CYB5; MCB5  [Similar Products]
NCBI Summary for CYB5A
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Comments for CYB5A
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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