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anti-DAB2 antibody :: Rabbit DAB2 Polyclonal Antibody

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Catalog # MBS768460
Unit / Price
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  0.1 mg  /  $265 +1 FREE 8GB USB
Product Name

DAB2, Polyclonal Antibody

Popular Item
Full Product Name

DAB2 Rabbit Polyclonal

Product Synonym Names
DAB2, Disabled homolog 2, DOC 2, DOC2, FLJ26626
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601236
3D Structure
ModBase 3D Structure for P98082
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Human, Mouse ,Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
Purification: Immunogen affinity purified
Purity: > or =95% as determined by SDS-PAGE
Form/Format
Liquid
Immunogen
Disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Preparation and Storage
Store at -20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-DAB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAB2 antibody
Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR.
Applications Tested/Suitable for anti-DAB2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-DAB2 antibody
WB : 1:500-1:5000
IP : 1:1000-1:10000
IF : 1:20-1:200

Immunofluorescent of anti-DAB2 antibody
Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
anti-DAB2 antibody Immunofluorescent image
SDS-PAGE of anti-DAB2 antibody
HeLa cells were subjected to SDS PAGE followed by western blot with MBS768460 (DAB2 antibody) at dilution of 1:1000
anti-DAB2 antibody SDS-PAGE image
NCBI/Uniprot data below describe general gene information for DAB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96 kDa
NCBI Official Full Name
disabled homolog 2 isoform 2
NCBI Official Synonym Full Names
DAB2, clathrin adaptor protein
NCBI Official Symbol
NCBI Official Synonym Symbols
DOC2; DOC-2
  [Similar Products]
NCBI Protein Information
disabled homolog 2
UniProt Protein Name
Disabled homolog 2
UniProt Synonym Protein Names
Adaptor molecule disabled-2; Differentially expressed in ovarian carcinoma 2; DOC-2; Differentially-expressed protein 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
DOC2; DOC-2  [Similar Products]
UniProt Entry Name
DAB2_HUMAN
NCBI Summary for DAB2
This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for DAB2
DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: clathrin coated vesicle membrane; clathrin-coated vesicle; coated pit; focal adhesion; intracellular membrane-bound organelle; lysosomal membrane; nucleolus; plasma membrane

Molecular Function: protein binding; protein C-terminus binding; SMAD binding

Biological Process: apoptosis; cell proliferation; integrin-mediated signaling pathway; leading edge cell differentiation; multicellular organismal development; negative regulation of apoptosis; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; positive regulation of endocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; protein transport; receptor-mediated endocytosis; Wnt receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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