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anti-DSG2 antibody :: Rabbit Desmoglein 2 Polyclonal Antibody

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Catalog # MBS175420
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Desmoglein 2 (DSG2), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Anti-Desmoglein 2, DSG2

Product Synonym Names
Desmoglein-2; desmoglein 2; ARVC 10 antibody; ARVC10 antibody; ARVD 10 antibody; ARVD10 antibody; Cadherin family member 5 antibody; CDHF 5 antibody; CDHF5 antibody; CMD1BB antibody; Desmoglein 2 antibody; Desmoglein-2 antibody; Desmoglein2 antibody; DSG 2 antibody; DSG2 antibody; DSG2_HUMAN antibody; HDGC antibody; HDGC included antibody; MGC117034 antibody; MGC117036 antibody; MGC117037 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1118
OMIM
612877
3D Structure
ModBase 3D Structure for Q14126
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 2 (1104-1118aa STRVTKHSTVQHSYS), different from the related mouse and rat sequences by one amino acid.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant detection
MBS provides a series of assays reacted with primary antibodies. Antibody can be be supported by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC (P)
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.

Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-DSG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DSG2 antibody
Description: Rabbit IgG polyclonal antibody for Desmoglein-2(DSG2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.
Applications Tested/Suitable for anti-DSG2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-DSG2 antibody
WB:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Concluded Species: Ms

IHC-P:
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Concluded Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-DSG2 antibody
Anti-Desmoglein 2 antibody, MBS175420, Western blotting
All lanes: Anti Desmoglein 2 (MBS175420) at 0.5ug/ml
Lane 1: HT1080 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: SCG Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
anti-DSG2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DSG2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Desmoglein-2
NCBI Official Synonym Full Names
desmoglein 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HDGC; CDHF5; ARVC10; ARVD10; CMD1BB
  [Similar Products]
NCBI Protein Information
desmoglein-2; HDGC; cadherin family member 5
UniProt Protein Name
Desmoglein-2
UniProt Synonym Protein Names
Cadherin family member 5; HDGC
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CDHF5  [Similar Products]
UniProt Entry Name
DSG2_HUMAN
NCBI Summary for DSG2
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jul 2008]
UniProt Comments for DSG2
DSG2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10); also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Membrane protein, integral; Cell adhesion; Calcium-binding

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; cell surface; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; lateral plasma membrane

Molecular Function: calcium ion binding

Biological Process: maternal process involved in pregnancy; apoptosis; homophilic cell adhesion; response to progesterone stimulus; cell adhesion; cell structure disassembly during apoptosis

Disease: Cardiomyopathy, Dilated, 1bb; Arrhythmogenic Right Ventricular Dysplasia, Familial, 10
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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