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anti-DNAJB6 antibody :: Rabbit DNAJB6 Polyclonal Antibody

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Catalog # MBS9126113
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

DNAJB6, Polyclonal Antibody

Popular Item
Full Product Name

DNAJB6 Polyclonal Antibody

Product Synonym Names
DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 611332
3D Structure
ModBase 3D Structure for O75190
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human DNAJB6
Calculated Molecular Weight
36kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-DNAJB6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DNAJB6 antibody
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
Product Categories/Family for anti-DNAJB6 antibody
Applications Tested/Suitable for anti-DNAJB6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-DNAJB6 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100

Western Blot (WB) of anti-DNAJB6 antibody
Western blot analysis of extracts of various cell lines, using DNAJB6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-DNAJB6 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-DNAJB6 antibody
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody at dilution of 1:100 (40x lens).
anti-DNAJB6 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-DNAJB6 antibody
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody at dilution of 1:100 (40x lens).
anti-DNAJB6 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-DNAJB6 antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody at dilution of 1:100 (40x lens).
anti-DNAJB6 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-DNAJB6 antibody
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody at dilution of 1:100 (40x lens).
anti-DNAJB6 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-DNAJB6 antibody
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody at dilution of 1:100 (40x lens).
anti-DNAJB6 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-DNAJB6 antibody
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody. Blue: DAPI for nuclear staining.
anti-DNAJB6 antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-DNAJB6 antibody
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
anti-DNAJB6 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for DNAJB6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
326
NCBI Official Full Name
DnaJ homolog subfamily B member 6
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily B, member 6
NCBI Official Symbol
DNAJB6  [Similar Products]
NCBI Official Synonym Symbols
DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
  [Similar Products]
NCBI Protein Information
dnaJ homolog subfamily B member 6; DnaJ-like 2 protein; heat shock protein J2
UniProt Protein Name
DnaJ homolog subfamily B member 6
UniProt Synonym Protein Names
HHDJ1; Heat shock protein J2; HSJ-2; MRJ; MSJ-1
Protein Family
UniProt Gene Name
DNAJB6  [Similar Products]
UniProt Synonym Gene Names
HSJ2; MRJ; MSJ1; HSJ-2  [Similar Products]
UniProt Entry Name
DNJB6_HUMAN
NCBI Summary for DNAJB6
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
UniProt Comments for DNAJB6
DNAJB6: Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. Defects in DNAJB6 are the cause of limb-girdle muscular dystrophy type 1E (LGMD1E). An autosomal dominant myopathy characterized by adult onset of proximal muscle weakness, beginning in the hip girdle region and later progressing to the shoulder girdle region. There is evidence that LGMD1E is caused by dysfunction of isoform B (PubMed:22366786). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 7q36.3

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; Z disc; nucleus; cytosol

Molecular Function: ATPase activator activity; protein binding; heat shock protein binding; chaperone binding; unfolded protein binding

Biological Process: protein folding; positive regulation of ATPase activity; intermediate filament organization; negative regulation of caspase activity

Disease: Muscular Dystrophy, Limb-girdle, Type 1e
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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