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anti-EBF1 antibody :: Goat EBF1/COE1 Polyclonal Antibody

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Catalog # MBS421968
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

EBF1/COE1, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-EBF1/COE1 Antibody

Product Synonym Names
EBF1; early B-cell factor 1; COE1; EBF; O/E-1; OLF1; Collier, Olf and EBF transcription factor 1; early B-cell factor olfactory neuronal transcription factor 1; EBF1 antibody; early B-cell factor 1 antibody; COE1 antibody; EBF antibody; O/E-1 antibody; OLF1 antibody; Collier; Olf and EBF transcription factor 1 antibody; early B-cell factor olfactory neuronal transcription factor 1 antibody; EBF1 / COE1
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
HNQLPALANT SV
OMIM
164343
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1.
Epitope
Internal region
Note
This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-EBF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EBF1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-EBF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx.75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.
Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Western Blot (WB) of anti-EBF1 antibody
(0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-EBF1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for EBF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,059 Da
NCBI Official Full Name
transcription factor COE1 isoform 2
NCBI Official Synonym Full Names
early B-cell factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
EBF; COE1; OLF1; O/E-1
  [Similar Products]
NCBI Protein Information
transcription factor COE1
UniProt Protein Name
Transcription factor COE1
UniProt Synonym Protein Names
Early B-cell factor
UniProt Gene Name
UniProt Synonym Gene Names
COE1; EBF; O/E-1; OE-1  [Similar Products]
UniProt Entry Name
COE1_HUMAN
UniProt Comments for EBF1
EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; protein binding

Biological Process: multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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