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anti-eIF2alpha antibody :: Rabbit eIF2alpha Polyclonal Antibody

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Catalog # MBS9407695
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

eIF2alpha, Polyclonal Antibody

Popular Item
Also Known As

eIF2alpha (Phospho-Ser51) Antibody

Product Synonym Names
eIF2a (Phospho-Ser51) Antibody; Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
315
OMIM
603907
3D Structure
ModBase 3D Structure for P05198
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.
Modification
Phospho-Ser51
Target Name
eIF2a
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Other Notes
Small volumes of anti-eIF2alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-eIF2alpha antibody
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Product Categories/Family for anti-eIF2alpha antibody
Applications Tested/Suitable for anti-eIF2alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-eIF2alpha antibody
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western Blot (WB) of anti-eIF2alpha antibody
Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-eIF2alpha antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) Antibody (left) or the same antibody preincubated with blocking peptide #51279 (right).
anti-eIF2alpha antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-eIF2alpha antibody
Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-eIF2alpha antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
36,112 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol
EIF2S1  [Similar Products]
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
UniProt Gene Name
EIF2S1  [Similar Products]
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha  [Similar Products]
UniProt Entry Name
IF2A_HUMAN
NCBI Summary for eIF2alpha
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Comments for eIF2alpha
eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: Translation initiation; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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