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anti-ERBB2 antibody :: Chicken erbB-2 Polyclonal Antibody

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Catalog # MBS150076
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

erbB-2 (ERBB2), Polyclonal Antibody

Also Known As

erbB-2 Antibody

Product Synonym Names
erbB-2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; MLN19; NEU; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
Antibody/Peptide Pairs
erbB-2 peptide (MBS152625) is used for blocking the activity of erbB-2 antibody (MBS150076)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1255
OMIM
phenotype 613659
3D Structure
ModBase 3D Structure for P04626
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Purity/Purification
erbB-2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
erbB-2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human erbB-2.
Buffer
erbB-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
erbB-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-ERBB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ERBB2 antibody
erbB-2 Antibody: The ErbB family consists of four closely related tyrosine kinase receptors that act as potent mediators of normal cell growth and development. Aberrant expression or function of one or more of these receptors can play a major role in the development and evolution of cancer. ErbB-2, also known as HER2, has been implicated in the evolution of both breast and gastric cancers, and is evident in other cancer types such as ovarian and salivary gland tumors. ErbB-2 possesses an active tyrosine kinase domain, but no direct ligand has been identified yet. ErbB-2 is the preferred binding partner to the other members of the ErbB family and is thought to act primarily through the Ras-MAPK, PI3k-PKB/Akt, and PLC-PKC signaling pathways. Numerous anti-cancer strategies have been employed against erbB-2, such as antibody-based therapies to prevent ligand binding or receptor activation through dimerization, antibody-dependent cell mediated cytotoxicity, in addition to direct kinase inhibition to prevent molecular activation/downstream signaling.
Applications Tested/Suitable for anti-ERBB2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-ERBB2 antibody
erbB-2 antibody can be used for detection of erbB-2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-ERBB2 antibody
Western blot analysis of erbB-2 in rat liver tissue lysate with erbB-2 antibody at (A) 1 and (B) 2 μg/mL.
anti-ERBB2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ERBB2 antibody
Immunohistochemistry of erbB-2 in human liver tissue with erbB-2 antibody at 2.5 μg/mL.
anti-ERBB2 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-ERBB2 antibody
Immunofluorescence of ERBB2 in human liver tissue with ERBB2 antibody at 20 μg/mL.
anti-ERBB2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ERBB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,253 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform a
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2  [Similar Products]
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
  [Similar Products]
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Synonym Protein Names
Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
UniProt Gene Name
ERBB2  [Similar Products]
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19  [Similar Products]
UniProt Entry Name
ERBB2_HUMAN
NCBI Summary for ERBB2
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for ERBB2
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Oncoprotein; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein dimerization activity; protein C-terminus binding; identical protein binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; ATP binding

Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; wound healing; positive regulation of translation; protein amino acid autophosphorylation; heart development; motor axon guidance; signal transduction; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; negative regulation of immature T cell proliferation in the thymus; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; regulation of angiogenesis; positive regulation of cell growth; peripheral nervous system development; cell proliferation; innate immune response; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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