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anti-FAK antibody :: Rabbit FAK Polyclonal Antibody

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Catalog # MBS9503025
Unit / Price
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  0.05 mg  /  $245 +1 FREE 8GB USB
  0.1 mg  /  $335 +1 FREE 8GB USB
Western Blot (WB)
Product Name

FAK, Polyclonal Antibody

Popular Item
Full Product Name

FAK (Phospho-Tyr397) Antibody

Product Synonym Names
FADK 1; FAK1; Focal adhesion kinase 1; pp125FAK; Protein- tyrosine kinase 2; PTK2
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
L13616 mRNA
3D Structure
ModBase 3D Structure for Q05397
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
FAK (Phospho-Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:Y397; M:Y397; R:Y397
Immunogen
The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.
Immunogen Range
364-413
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-FAK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FAK antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-FAK antibody
WB: 1:500~1:1000
ELISA: 1:5000

Western Blot (WB) of anti-FAK antibody
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.
anti-FAK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for FAK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
119 kDa
NCBI Official Full Name
focal adhesion kinase 1 isoform c
NCBI Official Synonym Full Names
protein tyrosine kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
  [Similar Products]
NCBI Protein Information
focal adhesion kinase 1
UniProt Protein Name
Focal adhesion kinase 1
UniProt Synonym Protein Names
Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
UniProt Gene Name
UniProt Synonym Gene Names
FAK; FAK1; FADK 1; FRNK; PPP1R71  [Similar Products]
NCBI Summary for FAK
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
UniProt Comments for FAK
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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