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anti-WDFY1 antibody :: Goat FENS1/WDFY1 Polyclonal Antibody

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Catalog # MBS420558
Unit / Price
  0.1 mg  /  $225 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

FENS1/WDFY1, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-FENS1/WDFY1 Antibody

Product Synonym Names
WDFY1; WDF1; FENS-1; KIAA1435; WD repeat and FYVE domain containing 1; WD40 and FYVE domain containing 1; phosphoinositide-binding protein SR1; ZFYVE17; WDFY1 antibody; WDF1 antibody; FENS-1 antibody; KIAA1435 antibody; WD repeat and FYVE domain containing 1 antibody; WD40 and FYVE domain containing 1 antibody; phosphoinositide-binding protein SR1 antibody; ZFYVE17 antibody; FENS1 / WDFY1
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
410
Immunogen Sequence
VVGCSLATGF SPH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence VVGCSLATGFSPH, from the C Terminus of the protein sequence according to NP_065881.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-WDFY1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WDFY1 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-WDFY1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave no signal but low background in Human Liver and HepG2 lysates at up to 1ug/ml.

Immunohistochemistry (IHC) of anti-WDFY1 antibody
(4ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
anti-WDFY1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for WDFY1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,324 Da
NCBI Official Full Name
WD repeat and FYVE domain-containing protein 1
NCBI Official Synonym Full Names
WD repeat and FYVE domain containing 1
NCBI Official Symbol
WDFY1  [Similar Products]
NCBI Official Synonym Symbols
WDF1; FENS-1; ZFYVE17
  [Similar Products]
NCBI Protein Information
WD repeat and FYVE domain-containing protein 1
UniProt Protein Name
WD repeat and FYVE domain-containing protein 1
UniProt Synonym Protein Names
FENS-1; Phosphoinositide-binding protein 1; WD40- and FYVE domain-containing protein 1; Zinc finger FYVE domain-containing protein 17
UniProt Gene Name
WDFY1  [Similar Products]
UniProt Synonym Gene Names
KIAA1435; WDF1; ZFYVE17  [Similar Products]
UniProt Entry Name
WDFY1_HUMAN
NCBI Summary for WDFY1
The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain. [provided by RefSeq, Jan 2015]
UniProt Comments for WDFY1
WDFY1: Binds PtdIns3P in vitro with high specificity over other phosphoinositides.

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: cytosol; early endosome; nucleus

Molecular Function: phosphatidylinositol binding; protein binding; zinc ion binding

Biological Process: positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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